Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	297,509	G→C	G30G (GGC→GGG)	paoB ←	aldehyde oxidoreductase FAD‑binding subunit PaoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	297,509	0	G	C	100.0%	98.5 / NA	29	G30G (GGC→GGG)	paoB	aldehyde oxidoreductase FAD‑binding subunit PaoB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (13/16);  total (13/16)

ATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAA  >  NZ_CP009273/297428‑297589                                                                                  |                                                                                 aTCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGAt                                                                          >  2:756368/1‑90 (MQ=255)      gCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAAtt                                                                     >  2:268602/1‑90 (MQ=255)             gCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGcc                                                              <  2:129355/90‑1 (MQ=255)                gTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCggg                                                           >  2:78694/1‑90 (MQ=255)                 ttCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGt                                                          >  1:593351/1‑90 (MQ=255)                        cGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCt                                                   >  1:20893/1‑90 (MQ=255)                        cGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCt                                                   <  1:837750/90‑1 (MQ=255)                         gATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTg                                                  <  2:385166/90‑1 (MQ=255)                         gATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTg                                                  <  1:78694/90‑1 (MQ=255)                               gtgggtggGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCt                                            >  1:673892/1‑90 (MQ=255)                                tgggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTa                                           <  1:531077/90‑1 (MQ=255)                                 gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTg                                                  <  1:305044/82‑1 (MQ=255)                                 gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTg                                                  >  2:305044/1‑82 (MQ=255)                                 gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTaa                                          >  1:658702/1‑90 (MQ=255)                                 gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTaa                                          >  1:432843/1‑90 (MQ=255)                                   gtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGt                                        >  2:719241/1‑90 (MQ=255)                                   gtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGt                                        >  2:814527/1‑90 (MQ=255)                                        cGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTgccgc                                   >  2:456519/1‑90 (MQ=255)                                         gTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTgccgcc                                  <  1:652307/90‑1 (MQ=255)                                         gTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTgccgcc                                  <  2:432843/90‑1 (MQ=255)                                                tttCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTg                           <  1:814527/90‑1 (MQ=255)                                                tttCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTg                           <  2:20893/90‑1 (MQ=255)                                                 ttCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTgg                          <  1:899203/90‑1 (MQ=255)                                                           atcaGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACt                <  1:456519/90‑1 (MQ=255)                                                           atcaGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACt                <  1:452115/90‑1 (MQ=255)                                                           atcaGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACt                <  2:756706/90‑1 (MQ=255)                                                            tcaGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTc               <  2:991638/90‑1 (MQ=255)                                                                  ccAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCAt         <  2:424063/90‑1 (MQ=255)                                                                         aTTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTaaa  >  2:25588/1‑90 (MQ=255)                                                                                  |                                                                                 ATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAA  >  NZ_CP009273/297428‑297589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑