Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,849,816	T→C	E117G (GAA→GGA)	ygbA ←	nitrous oxide‑stimulated promoter family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,849,816	0	T	C	100.0%	116.3 / NA	34	E117G (GAA→GGA)	ygbA	nitrous oxide‑stimulated promoter family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/13);  total (21/13)

CACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTA  >  NZ_CP009273/2849732‑2849894                                                                                     |                                                                               cACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCAttg                                                                           >  1:487286/1‑88 (MQ=255) cACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGa                                                                           >  1:320830/1‑90 (MQ=255) cACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGa                                                                           >  1:714865/1‑90 (MQ=255)  aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAg                                                                          >  2:384943/1‑90 (MQ=255)         aaGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGtttttt                                                                   <  1:70596/90‑1 (MQ=255)              gCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGAc                                                              >  1:729471/1‑90 (MQ=255)                 gggAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGat                                                           >  1:450093/1‑90 (MQ=255)                             aTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCa                                               <  1:906884/90‑1 (MQ=255)                             aTCGGCATTGATTAAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCa                                               >  2:52363/1‑90 (MQ=255)                             aTCGGCATTGATTAAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCa                                               <  1:52363/90‑1 (MQ=255)                                 gCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTc                                           >  2:986528/1‑90 (MQ=255)                                           aaCTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGa                                 >  1:534315/1‑90 (MQ=255)                                                cAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCtt                            >  1:443678/1‑90 (MQ=255)                                                    ttGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCa                        <  1:986528/90‑1 (MQ=255)                                                       ccGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCg                     >  2:309811/1‑90 (MQ=255)                                                           aCAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatga                 <  1:449806/90‑1 (MQ=255)                                                              gATCTGCCTGTCCGGCATGCTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatgagat              <  1:525901/90‑1 (MQ=255)                                                              gATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatgagat              >  2:733752/1‑90 (MQ=255)                                                              gATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgatgagat              >  1:30584/1‑90 (MQ=255)                                                                    ccTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa        <  2:322006/90‑1 (MQ=255)                                                                    ccTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa        <  2:534315/90‑1 (MQ=255)                                                                    ccTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa        >  2:605804/1‑90 (MQ=255)                                                                    ccTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa        >  2:843375/1‑90 (MQ=255)                                                                    ccTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa        <  2:714865/90‑1 (MQ=255)                                                                     cTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCAc       <  1:439724/90‑1 (MQ=255)                                                                     cTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCAc       <  1:287110/90‑1 (MQ=255)                                                                        tCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCa                                               <  1:916103/47‑1 (MQ=255)                                                                        tCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCa                                               >  2:916103/1‑47 (MQ=255)                                                                        tCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt    >  1:166053/1‑90 (MQ=255)                                                                        tCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt    >  2:375727/1‑90 (MQ=255)                                                                        tCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt    >  2:683045/1‑90 (MQ=255)                                                                        tCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt    >  1:73825/1‑90 (MQ=255)                                                                        tCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt    >  1:656479/1‑90 (MQ=255)                                                                          cGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTa  <  2:954550/90‑1 (MQ=255)                                                                                     |                                                                               CACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTA  >  NZ_CP009273/2849732‑2849894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑