| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 319,062 | A→T | P338P (CCA→CCT) | ykgF → | LutB/LldF family L‑lactate oxidation iron‑sulfur protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 319,062 | 0 | A | T | 96.2% | 74.3 / NA | 27 | P338P (CCA→CCT) | ykgF | LutB/LldF family L‑lactate oxidation iron‑sulfur protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base T (11/14); minor base C (1/0); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAG > NZ_CP009273/318976‑319147 | ctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCTggg < 1:210085/90‑1 (MQ=255) tgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCTGGGc < 1:366046/90‑1 (MQ=255) cgcTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCTGGGCCa < 2:113606/90‑1 (MQ=255) gTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCTGGGCCAATTg > 2:637711/1‑90 (MQ=255) gTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGGCATGGGTATGGGTCTTTTTTTTCTGGGGCCATTg > 1:159615/1‑90 (MQ=255) cGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTTTTCTTGGCCCATTGGTGCg > 1:310383/1‑90 (MQ=255) cGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGGTATGGGTCTTTTTTTCCCGGGCCCATTTGTGCgggggt > 1:533399/1‑86 (MQ=255) ttGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCTGGGCCAATTGGTGCGGTGATTTCTcc < 1:623842/90‑1 (MQ=255) tGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCTGGGCCAATTGGTGCGGGGATTTCTccc > 2:124558/1‑89 (MQ=255) cTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCTGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTggcg < 1:52283/90‑1 (MQ=255) ttGTCCGGCATATCGCCATATTGGCGGTCATGGGTATGGCTCTATTTATCCTGGGCCAATTGGGGGGGGGATTTCTCCGCTACTTggcgg > 2:434472/1‑90 (MQ=255) gTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCTGGGCCAATTGGTGCGGTGATTtctc > 2:140818/1‑75 (MQ=255) gTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCTGGGCCAATTGGTGCGGTGATTtctc < 1:140818/75‑1 (MQ=255) ggCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTTTTCTTGGCCCATTTGGGCGGGGGTTTTTCCCCCACTTTGCGGCTAtaa > 1:214802/1‑90 (MQ=255) tCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCTGGGCCAATTGGTGCGGGGGTTTCTCCGCTACTTGGCGGCTAtaaagattt > 2:795943/1‑90 (MQ=255) tGGCGGTCATGGATATGGCTCTATTTATCCTGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagattt < 2:369439/90‑1 (MQ=255) tGGCGGTCATGGATATGGCTCTATTTATCCTGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagattt < 2:638321/90‑1 (MQ=255) aTGGATATGGCTCTATTTATCCTGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACg > 2:524442/1‑90 (MQ=255) atatGGCTCTATTTATCCTGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTg < 2:707404/90‑1 (MQ=255) atGGCTCTATTTATCCTGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGct < 2:214802/90‑1 (MQ=255) tGGCTCTATTTATCCTGGGCCAATTGGTGCGGTGATTTCTCCGc > 2:392683/1‑44 (MQ=255) tGGCTCTATTTATCCTGGGCCAATTGGTGCGGTGATTTCTCCGc < 1:392683/44‑1 (MQ=255) tGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGctc > 2:986997/1‑90 (MQ=255) ctATTTATCCTGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTAt > 1:454562/1‑90 (MQ=255) ttATCCTGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGcac < 2:404759/90‑1 (MQ=255) aTCCTGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAg < 2:54969/90‑1 (MQ=255) tCCTGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGct < 2:584024/77‑1 (MQ=255) | CTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAG > NZ_CP009273/318976‑319147 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |