Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,273,721	A→G	E49G (GAA→GGA)	agaV →	PTS N‑acetylgalactosamine transporter subunit IIB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,273,721	0	A	G	100.0%	76.4 / NA	23	E49G (GAA→GGA)	agaV	PTS N‑acetylgalactosamine transporter subunit IIB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/12);  total (11/12)

TTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAG  >  NZ_CP009273/3273634‑3273806                                                                                        |                                                                                      ttCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGgaa                                                                                     <  2:837270/90‑1 (MQ=255) ttCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGgaa                                                                                     <  2:679077/90‑1 (MQ=255)       gggTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTAc                                                                               <  2:134752/90‑1 (MQ=255)        ggTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACt                                                                              >  2:807463/1‑90 (MQ=255)        ggTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACt                                                                              >  2:440792/1‑90 (MQ=255)                 tGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAgg                                                                     >  2:603551/1‑90 (MQ=255)                      gggCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCg                                                                >  2:303836/1‑90 (MQ=255)                            aTCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTAc                                                          <  2:556627/90‑1 (MQ=255)                            aTCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTAc                                                          <  2:950659/90‑1 (MQ=255)                            aTCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTAc                                                          <  2:8902/90‑1 (MQ=255)                            aTCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTAc                                                          <  1:242928/90‑1 (MQ=255)                                     tGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTAc                                                          <  1:890270/81‑1 (MQ=255)                                     tGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTAc                                                          >  2:890270/1‑81 (MQ=255)                                       gTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACg                                               <  1:779888/90‑1 (MQ=255)                                                gATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCaaaaa                                      >  1:893759/1‑90 (MQ=255)                                                      gTTGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATc                                <  2:632932/90‑1 (MQ=255)                                                        tGCCGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGa                              >  2:720996/1‑90 (MQ=255)                                                          ccGAAGATCCGGTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGaca                            <  1:603551/90‑1 (MQ=255)                                                                    ggTACAACAAAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATcg                  >  2:880195/1‑90 (MQ=255)                                                                          acaaAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGacaa                           <  2:755755/75‑1 (MQ=255)                                                                          acaaAACCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGacaa                           >  1:755755/1‑75 (MQ=255)                                                                               aCCTGATGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATc       >  1:275284/1‑90 (MQ=255)                                                                                  tGATGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGc                                              <  1:913439/48‑1 (MQ=255)                                                                                  tGATGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGc                                              >  2:913439/1‑48 (MQ=255)                                                                                    aTGGGAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAg  <  1:630132/90‑1 (MQ=255)                                                                                        |                                                                                      TTCAATGGGTCGGATTTGCGGGGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAG  >  NZ_CP009273/3273634‑3273806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑