| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,576,634 | A→G | R79R (CGA→CGG) | BW25113_RS17825 → | YrhA family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,576,634 | 0 | A | G | 93.8% | 43.4 / ‑3.1 | 16 | R79R (CGA→CGG) | BW25113_RS17825 | YrhA family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (6/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01 | |||||||||||
AATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAA > NZ_CP009273/3576548‑3576719 | aaTGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGgtta > 2:512539/1‑90 (MQ=255) aTTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCg < 1:300078/90‑1 (MQ=255) aTTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCg > 2:122941/1‑90 (MQ=255) tCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGAt < 2:378348/90‑1 (MQ=255) tttttAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTc > 1:140780/1‑90 (MQ=255) tttttAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTc > 2:393104/1‑90 (MQ=255) ttAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAg < 2:952963/90‑1 (MQ=255) tCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTc < 1:98972/90‑1 (MQ=255) cAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCa < 1:300005/90‑1 (MQ=255) gATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTaaa > 1:680854/1‑90 (MQ=255) atggatggATTCGAAGTTAATGGATTACGGTTATTTAGcc < 1:875591/40‑1 (MQ=39) atggatggATTCGAAGTTAATGGATTACGGTTATTTAGcc > 2:875591/1‑40 (MQ=39) atggatTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACTTTTTTGCCGTAAATGAATTTtata < 2:352293/90‑1 (MQ=255) aaGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAatg < 1:393104/90‑1 (MQ=255) tAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAAtgatga < 1:86547/90‑1 (MQ=255) tAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAAtgatga > 1:503257/1‑90 (MQ=255) tACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATaa < 1:133371/90‑1 (MQ=255) | AATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAA > NZ_CP009273/3576548‑3576719 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |