Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,896,621	A→G	D147D (GAT→GAC)	bglB ←	6‑phospho‑beta‑glucosidase BglB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,896,621	0	A	G	100.0%	128.9 / NA	42	D147D (GAT→GAC)	bglB	6‑phospho‑beta‑glucosidase BglB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/14);  total (28/14)

TGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCTT  >  NZ_CP009273/3896539‑3896707                                                                                   |                                                                                       tGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGa                                                                                 >  1:234624/1‑90 (MQ=255)     gATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGc                                                                             <  1:120793/90‑1 (MQ=255)     gATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGc                                                                             <  2:801111/90‑1 (MQ=255)     gATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGc                                                                             <  2:589552/90‑1 (MQ=255)         tCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGa                                                                         >  1:294808/1‑90 (MQ=255)                          tAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTa                                                        >  1:227639/1‑90 (MQ=255)                                cACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGttttt                                                  >  2:286531/1‑90 (MQ=255)                                cACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGttttt                                                  >  1:384861/1‑90 (MQ=255)                                       ggtgggTGGCGAGAAAAGCCGGGGGGGGCGTAATGCTCGAGGGGGTCGAGGCCCGCTCGTTTACCCCAACCGCCGTAGTTTTTCACCAGc                                           <  1:635992/86‑1 (MQ=255)                                        tttGGTAGCGAGAAAAGCCCGGGGGGGCGAAATGCTCGAGGTGGTCGATGCCCGCTCGATTACCCAAACCGCCGTAGTTTTTCACCAGcc                                          <  2:575199/89‑1 (MQ=255)                                        gTTGGTAGCGAGAAAAGCCCGGGGGGGCGTAATGCTCGAAGTGGTCGATGCCCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGcc                                          <  2:167352/90‑1 (MQ=255)                                           ggTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCat                                       >  2:618449/1‑90 (MQ=255)                                           ggTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCat                                       >  1:354227/1‑90 (MQ=255)                                           ggTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCat                                       >  1:244776/1‑90 (MQ=255)                                            gTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCata                                      >  1:468421/1‑90 (MQ=255)                                            gTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCata                                      >  2:508983/1‑90 (MQ=255)                                             tAGCGAGAAAAGCCCGTGGGGGCGAAATGCTAGAAGTGGTCGATGCCCCCTCGATTACCCCAACCGCCGTAGTTTTTCACCAGCCCatat                                     <  2:777326/90‑1 (MQ=255)                                              aGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATg                                    >  2:767716/1‑90 (MQ=255)                                                cGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGc                                  >  2:352979/1‑90 (MQ=255)                                                  aGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCAt                                >  1:795645/1‑90 (MQ=255)                                                   gTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCAtt                               >  2:288741/1‑90 (MQ=255)                                                         cccGGGCGGGTTTATTGCTGGAAGTGGTCGTTGCCCCCTCGATTACCCCACCCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTaa                         <  2:354489/89‑1 (MQ=255)                                                         cccGGGCGGGCGTAATCCTCGAAGGGGTCGATGCCCCCTCGATTACCCCACCCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTaa                         <  1:945854/89‑1 (MQ=255)                                                           cGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATg                       >  1:956728/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:343383/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  1:506970/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:517859/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:802394/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:487755/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  1:736249/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:290783/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  1:541960/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  1:664881/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:1000434/1‑90 (MQ=255)                                                            gTGCGGGCGTAATGCTCGAAGTGGTCGATAACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:781288/1‑90 (MQ=255)                                                             tGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATggg                     >  1:466351/1‑90 (MQ=255)                                                                gggCGTATTGCTCGAGGTGGTCGATGACCGCTCGATTACCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATa                  <  2:227639/90‑1 (MQ=255)                                                                gggCGTAATGCTCGAAGTGGTCGATGCCCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATa                  <  2:403420/90‑1 (MQ=255)                                                                gggCGTAATGCCCGAAGTGGTCGATGCCCGCTCGATTACCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATa                  <  2:12927/90‑1 (MQ=255)                                                                              aaGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGc    >  1:181476/1‑90 (MQ=255)                                                                                gTGGTCGATGCCCGCTCGATTACCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCtt  <  2:908727/90‑1 (MQ=255)                                                                                gTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCtt  <  2:506970/90‑1 (MQ=255)                                                                                   |                                                                                       TGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCTT  >  NZ_CP009273/3896539‑3896707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑