| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,065,206 | A→T | L269Q (CTG→CAG) | yihT ← | sulfofructosephosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,065,206 | 0 | A | T | 95.0% | 138.9 / ‑0.4 | 40 | L269Q (CTG→CAG) | yihT | sulfofructosephosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (16/22); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01 | |||||||||||
TATCCTCATCTTAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCAGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCC > NZ_CP009273/4065123‑4065292 | tATCCTCATCTTAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCa < 1:472663/90‑1 (MQ=255) tCATCTTAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCg > 2:645608/1‑90 (MQ=255) tCATCTTAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCg > 2:521367/1‑90 (MQ=255) tCATCTTAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCg > 1:627273/1‑90 (MQ=255) cATCTTAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGt > 2:872397/1‑90 (MQ=255) tCTTAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTg < 2:384992/90‑1 (MQ=255) tCTTAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTg < 2:165007/90‑1 (MQ=255) ttAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTc < 1:332364/90‑1 (MQ=255) tAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCAGCATCAGCTCGTTGTCt > 1:429716/1‑90 (MQ=255) tAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGCCACGCTGCATCAGCTCGTTGTCt > 2:919805/1‑90 (MQ=255) ggcgTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTa < 1:958349/90‑1 (MQ=255) gcgTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTaa > 1:221751/1‑90 (MQ=255) gTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAAc > 1:325639/1‑90 (MQ=255) gTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAAc > 2:266400/1‑90 (MQ=255) gCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACcgacg < 1:182106/90‑1 (MQ=255) gCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACcgacg < 2:1941/90‑1 (MQ=255) tcatTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCg < 1:363514/90‑1 (MQ=255) tcatTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCg < 1:603005/90‑1 (MQ=255) tcatTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCg < 1:322829/90‑1 (MQ=255) tcatTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCg < 1:737967/90‑1 (MQ=255) tcatTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCg < 1:919805/90‑1 (MQ=255) tcatTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCg < 2:811970/90‑1 (MQ=255) tcatTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCg < 2:803928/90‑1 (MQ=255) tcatTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCAGCATCAGCTCGTTGTCTGGTAAACCGACGACCg < 2:347719/90‑1 (MQ=255) tcgACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAATGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAg < 1:620148/90‑1 (MQ=255) tcgACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAg < 2:102339/90‑1 (MQ=255) atCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACg < 2:540058/90‑1 (MQ=255) tCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACgg > 1:131429/1‑90 (MQ=255) gttgttGTAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCa > 2:713492/1‑90 (MQ=255) tgttgtAATTTCGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAgg < 1:122277/90‑1 (MQ=255) cGGTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATgc > 2:59707/1‑90 (MQ=255) ggTGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATgcg < 2:5826/90‑1 (MQ=255) tGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATgc < 2:310540/87‑1 (MQ=255) tGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATgc > 1:310540/1‑87 (MQ=255) tGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGcgcc > 2:422636/1‑90 (MQ=255) tGCGCAAACGTCACGCTGCATCAGCTCGTTGTCTAGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGcgcc > 2:604061/1‑90 (MQ=255) gcgcAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGcgccc > 1:218395/1‑90 (MQ=255) gcgcAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGcgccc < 2:394556/90‑1 (MQ=255) gcAAACGTCACGCTGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGcgcccgc > 2:381889/1‑90 (MQ=255) cGCTGCATCAGCTCGTTGTCTGGTAACCCGACGCCCGATGCCCAGACTGCCCGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGcc < 1:563076/90‑1 (MQ=255) | TATCCTCATCTTAGCGGCGTTTAGCCATCATTTCGTCGACGATATCGCCAAGTTGTTGTAATTTCGGTGCGCAAACGTCACGCAGCATCAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCC > NZ_CP009273/4065123‑4065292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |