Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,153,816	T→C	S84P (TCA→CCA)	btuB →	TonB‑dependent vitamin B12 receptor BtuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,153,816	0	T	C	100.0%	97.0 / NA	30	S84P (TCA→CCA)	btuB	TonB‑dependent vitamin B12 receptor BtuB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/17);  total (13/17)

GATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGG  >  NZ_CP009273/4153756‑4153901                                                             |                                                                                      gatgTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGcc                                                          <  2:663136/90‑1 (MQ=255) gatgTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGcc                                                          <  1:101850/90‑1 (MQ=255)    gtgCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTTTTTGCGGTACAAATGCCAGt                                                       >  2:828546/1‑90 (MQ=255)     tgctgcGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTTAGGGCAGCTCCCATCTATTTTTTTTTTCGGGACAAATGCCAATc                                                      >  2:970458/1‑90 (MQ=255)      gctgcGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCa                                                     <  2:820730/90‑1 (MQ=255)      gctgcGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCa                                                     <  2:408482/90‑1 (MQ=255)         gcgcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCCTCTATTTTTTTTTGCGGTACAAAAGCCCGTCAtgt                                                  >  1:388492/1‑90 (MQ=255)         gcgcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCCTCTATTTTTTTTTGCGGTACAAAAGCCAGTCAtgt                                                  >  1:930855/1‑90 (MQ=255)         gcgcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCAtgt                                                  >  2:437920/1‑90 (MQ=255)         gcgcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGGTCAGGTCCCCCCCCCTCGATTTTTATTCCCGGGACAAATGCCAGTctagt                                                  >  1:739393/1‑86 (MQ=255)            ccGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGtt                                               <  1:437920/90‑1 (MQ=255)                         gTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGAt                                  <  2:534471/90‑1 (MQ=255)                         gTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGAt                                  <  1:815826/90‑1 (MQ=255)                         atCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGAt                                  <  2:268961/89‑1 (MQ=255)                              tatCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGt                             <  1:621006/90‑1 (MQ=255)                              tatCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGt                             <  1:420492/90‑1 (MQ=255)                                tCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTAc                           <  2:478728/90‑1 (MQ=255)                                tCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTAc                           <  1:370950/90‑1 (MQ=255)                                       aaaCGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGaa                    >  2:275645/1‑90 (MQ=255)                                       aaaCGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGGACAAAAGCCCGTCATGTGTTGGGGTTAATTTATGGGGTACGCCTGaa                    >  1:165512/1‑90 (MQ=255)                                       aaaCGGCGGGTCAGGTCCGCTCCCATCTATTTTTATTCGCGGTACAAAAACCCGTCATTGGTTGGGGTTGATTGATGGCGGACGCCTGaa                    >  1:762364/1‑90 (MQ=255)                                         aCGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATc                  <  2:182679/90‑1 (MQ=255)                                         aCGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATc                  <  2:90887/90‑1 (MQ=255)                                            gcggTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTgg               >  2:399067/1‑90 (MQ=255)                                             cggTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGc              >  2:940341/1‑90 (MQ=255)                                             cggTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGc              <  1:161080/90‑1 (MQ=255)                                                       cAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGt    <  1:275645/90‑1 (MQ=255)                                                         gCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCa                                                     <  1:84412/39‑1 (MQ=39)                                                         gCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCa                                                     >  2:84412/1‑39 (MQ=39)                                                         gCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTgg  >  2:328958/1‑90 (MQ=255)                                                             |                                                                                      GATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGG  >  NZ_CP009273/4153756‑4153901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑