Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,367,563	T→C	intergenic (‑24/+65)	blc ← / ← ampC	lipocalin Blc/BlaEC family class C beta‑lactamase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,367,563	0	T	C	100.0%	128.4 / NA	37	intergenic (‑24/+65)	blc/ampC	lipocalin Blc/BlaEC family class C beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/26);  total (11/26)

AGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAA  >  NZ_CP009273/4367477‑4367647                                                                                       |                                                                                     agaACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGTGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTa                                                                                   <  1:201069/90‑1 (MQ=255) agaACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTa                                                                                   <  1:198653/90‑1 (MQ=255) agaACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTa                                                                                   <  1:102058/90‑1 (MQ=255) agaACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTa                                                                                   <  2:659771/90‑1 (MQ=255) agaACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTa                                                                                   <  2:330098/90‑1 (MQ=255) agaACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTa                                                                                   <  1:675221/90‑1 (MQ=255) agaACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTa                                                                                   <  1:862155/90‑1 (MQ=255) agaACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGCGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTa                                                                                   <  2:113668/90‑1 (MQ=255)    aCTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAgt                                                                                >  1:729570/1‑90 (MQ=255)     cTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAgtg                                                                               >  2:234169/1‑90 (MQ=255)               cGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAg                                                                     >  1:272029/1‑90 (MQ=255)               cGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAg                                                                     >  1:38830/1‑90 (MQ=255)               cGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAg                                                                     >  2:71422/1‑90 (MQ=255)                         tGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAgt                                                                                >  1:144379/1‑69 (MQ=255)                         tGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAgt                                                                                <  2:144379/69‑1 (MQ=255)                          gCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGc                                                          <  2:971504/90‑1 (MQ=255)                           cAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCg                                                         >  1:62134/1‑90 (MQ=255)                            aGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCgg                                                        <  2:77267/90‑1 (MQ=255)                               tGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGgaga                                                     >  1:924906/1‑90 (MQ=255)                                                  gggAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGa                                  >  2:862412/1‑90 (MQ=255)                                                   ggAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGAc                                 <  2:203489/90‑1 (MQ=255)                                                        aGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGAt                            <  1:925564/90‑1 (MQ=255)                                                        aGGCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGAt                            <  2:507987/90‑1 (MQ=255)                                                         ggCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGc                                                                    <  1:677083/49‑1 (MQ=255)                                                         ggCGCATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGc                                                                    >  2:677083/1‑49 (MQ=255)                                                          gcgcATAAATGTTTCCTTACTGG‑TTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGa                         <  1:493615/90‑1 (MQ=255)                                                           cgcATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGa                         <  2:163224/90‑1 (MQ=255)                                                             cATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAAt                       <  1:328246/90‑1 (MQ=255)                                                             cATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAAt                       <  1:820760/90‑1 (MQ=255)                                                             cATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAAt                       <  1:448940/90‑1 (MQ=255)                                                             cATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAAt                       <  2:64856/90‑1 (MQ=255)                                                             cATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAAt                       <  2:641229/90‑1 (MQ=255)                                                             cATAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAAt                       <  1:578989/90‑1 (MQ=255)                                                              aTAAATGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAAtt                      >  2:278618/1‑90 (MQ=255)                                                                  aTGTTTCCTTACTGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTAc                  <  1:862412/90‑1 (MQ=255)                                                                       tCCTTACTGGTTTTTCCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTag             <  2:735790/90‑1 (MQ=255)                                                                              tGGTTTTTTCCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTa      <  1:614593/90‑1 (MQ=255)                                                                                  tttttcCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGaa  <  1:611516/90‑1 (MQ=255)                                                                                       |                                                                                     AGAACTGCAGGCAACGACCAGAAATGCAGCTGTCGCTGCGGCAACGAGAGGGAGCAGGCGCATAAATGTTTCCTTACTGGTTTTTTTCTAAGTGTAGATGACAGCAAGGAAAAGCGGAGAAAAGGTCCGAAAATTCGGACCCGATGGAATTTTACTGTAGAGCGTTAAGAA  >  NZ_CP009273/4367477‑4367647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑