Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,246,706	A→G	E62G (GAA→GGA)	dhaR →	dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,246,706	0	A	G	100.0%	79.6 / NA	24	E62G (GAA→GGA)	dhaR	dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/12);  total (12/12)

GTACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGGAATATATGGCACCGCGAGAGTGTGCGCTGTTTATCCTCGATGAAACCGCCTGCATT  >  NZ_CP009273/1246621‑1246773                                                                                      |                                                                    gTACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGAt                                                                 >  1:892147/1‑90 (MQ=255)  tACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATg                                                                >  1:288651/1‑90 (MQ=255)     cacaTCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCt                                                             <  2:928151/90‑1 (MQ=255)      acaTCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTg                                                            <  2:375567/90‑1 (MQ=255)       caTCAGGCCCAGGGCGTGACATTTGCCTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTgg                                                           >  2:876151/1‑90 (MQ=255)        aTCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCCGCGCTGGGAGATGCCTggg                                                          <  1:53616/90‑1 (MQ=255)         tCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGa                                                         >  2:68534/1‑90 (MQ=255)          cAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGaa                                                        <  1:68534/90‑1 (MQ=255)          cAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGaa                                                        <  1:708683/90‑1 (MQ=255)           aGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGAat                                                       >  1:737280/1‑90 (MQ=255)                      gTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGc                                                               >  2:693605/1‑71 (MQ=255)                      gTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGc                                                               <  1:693605/71‑1 (MQ=255)                      gTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGAATATATGGCACcg                                            >  1:923245/1‑90 (MQ=255)                      gTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGAATATATGGCACcg                                            >  2:380613/1‑90 (MQ=255)                      gTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGAATATATGGCACcg                                            >  2:34651/1‑90 (MQ=255)                             ttGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGAATATATGGCACCGCGAGAgt                                     >  1:741027/1‑90 (MQ=255)                                 ttCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGAATATATGGCACCGCGAGAGTGTgc                                 <  2:737280/90‑1 (MQ=255)                                   cTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGAATATATGGCACCGCGAGAGTGTgcgc                               <  2:403575/90‑1 (MQ=255)                                   cTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGAATATATGGCACCGCGAGAGTGTgcgc                               <  1:643941/90‑1 (MQ=255)                                   cTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGAATATATGGCACCGCGAGAGTGTgcgc                               <  1:443059/90‑1 (MQ=255)                                                       gCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGaa                                                        <  2:994139/45‑1 (MQ=255)                                                       gCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGaa                                                        >  1:994139/1‑45 (MQ=255)                                                       gCGATGCTGACGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGAATATATGGCACCGCGAGAGTGTGCGCTGTTTATCCTCGATGAAAcc           <  2:326700/90‑1 (MQ=255)                                                                aCGCTCGGGCAGGCTGCGCTGGGAGATGCCTGGGAATATATGGCACCGCGAGAGTGTGCGCTGTTTATCCTCGACGAAACCGCCTGCAtt  >  2:923422/1‑90 (MQ=255)                                                                                      |                                                                    GTACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGGAATATATGGCACCGCGAGAGTGTGCGCTGTTTATCCTCGATGAAACCGCCTGCATT  >  NZ_CP009273/1246621‑1246773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑