Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,531,275	G→A	L98L (CTG→TTG)	narW ←	nitrate reductase molybdenum cofactor assembly chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,531,275	0	G	A	100.0%	152.9 / NA	41	L98L (CTG→TTG)	narW	nitrate reductase molybdenum cofactor assembly chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/15);  total (26/15)

ATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAA  >  NZ_CP009273/1531191‑1531359                                                                                     |                                                                                     aTCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCa                                                                                 >  1:160462/1‑90 (MQ=255) aTCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCa                                                                                 >  2:380615/1‑90 (MQ=255) aTCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCa                                                                                 >  2:476025/1‑90 (MQ=255)        tttCGCCTGATCGTCAGGCAGTACGCTTAAATGCTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAc                                                                          <  2:46266/90‑1 (MQ=255)        tttCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAc                                                                          <  1:173340/90‑1 (MQ=255)        tttCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAc                                                                          <  2:754332/90‑1 (MQ=255)        tttCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAc                                                                          <  2:360730/90‑1 (MQ=255)        tttCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAc                                                                          <  2:320261/90‑1 (MQ=255)         ttCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAcc                                                                         <  1:957524/90‑1 (MQ=255)           cGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCtt                                                                       >  1:34737/1‑90 (MQ=255)                gATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCAt                                                                  >  2:135616/1‑90 (MQ=255)                           cgTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCcagc                                                       <  2:240778/89‑1 (MQ=255)                            gTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCcagca                                                      >  2:854829/1‑90 (MQ=255)                            gTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCcagca                                                      <  1:571515/90‑1 (MQ=255)                            gTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCcagca                                                      >  1:813358/1‑90 (MQ=255)                                gCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTc                                                  <  1:741732/90‑1 (MQ=255)                                    aaaTACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTccacc                                              >  1:977236/1‑90 (MQ=255)                                        aCTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTg                                          >  1:8728/1‑90 (MQ=255)                                           ccAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCTGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCt                                       >  2:250009/1‑90 (MQ=255)                                           ccAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCt                                       >  2:912879/1‑90 (MQ=255)                                                  cAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCcgcg                                >  2:454177/1‑90 (MQ=255)                                                  cAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCcgcg                                <  1:159204/90‑1 (MQ=255)                                                  cAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCcgcg                                >  2:96476/1‑90 (MQ=255)                                                     cGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATc                             <  2:160462/90‑1 (MQ=255)                                                       gTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGCCCACCATTGCCTGGCCGCGGTcgc                           >  1:800117/1‑90 (MQ=38)                                                              aTCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGGCCACCATTGCCTGGCCGCGATCGCggggccc                    >  1:432767/1‑86 (MQ=255)                                                                 gggCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCca                                                         <  1:281518/50‑1 (MQ=255)                                                                 gggCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCca                                                         >  2:281518/1‑50 (MQ=255)                                                                 gggCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGc                 >  2:204938/1‑90 (MQ=255)                                                                      gTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGaa            >  2:965323/1‑90 (MQ=255)                                                                       ttCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAAc           <  2:892713/90‑1 (MQ=255)                                                                           cGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGt       <  2:31270/90‑1 (MQ=255)                                                                               aaTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGa   <  1:900665/90‑1 (MQ=255)                                                                                aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa  >  1:170365/1‑90 (MQ=255)                                                                                aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa  >  1:681867/1‑90 (MQ=255)                                                                                aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa  >  1:310907/1‑90 (MQ=255)                                                                                aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa  >  1:677918/1‑90 (MQ=255)                                                                                aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa  >  2:175015/1‑90 (MQ=255)                                                                                aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa  >  1:865391/1‑90 (MQ=255)                                                                                aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa  >  1:903578/1‑90 (MQ=255)                                                                                aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGCTCGaa  >  1:236920/1‑90 (MQ=255)                                                                                     |                                                                                     ATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAA  >  NZ_CP009273/1531191‑1531359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑