Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,547,935	A→G	V54A (GTA→GCA)	adhP ←	alcohol dehydrogenase AdhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,547,935	0	A	G	100.0%	86.9 / NA	26	V54A (GTA→GCA)	adhP	alcohol dehydrogenase AdhP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

CCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGC  >  NZ_CP009273/1547849‑1548017                                                                                       |                                                                                   ccacgcTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTgcgc                                                                                 <  2:703573/90‑1 (MQ=255)                               aGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTaa                                                   >  1:345456/1‑90 (MQ=255)                               aGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTaa                                                   >  1:137050/1‑90 (MQ=255)                                ggTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCaaa                                                                 <  1:394834/75‑1 (MQ=255)                                ggTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCaaa                                                                 >  2:394834/1‑75 (MQ=255)                                       ccTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGa                                           <  2:270430/90‑1 (MQ=255)                                         tGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                                         <  2:912222/90‑1 (MQ=255)                                          ggACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCg                                        >  1:200888/1‑90 (MQ=255)                                             cccACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTa                                     <  2:650501/90‑1 (MQ=255)                                             cccACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTa                                     <  2:200888/90‑1 (MQ=255)                                               cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATg                                   >  1:257270/1‑90 (MQ=255)                                               cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATg                                   >  2:504621/1‑90 (MQ=255)                                               cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATg                                   >  2:48538/1‑90 (MQ=255)                                               cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGCTATg                                   >  2:200695/1‑90 (MQ=255)                                                   tCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACAt                               <  2:994545/90‑1 (MQ=255)                                                   tCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACAt                               <  1:398025/90‑1 (MQ=255)                                                     tGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATac                             <  2:256302/90‑1 (MQ=255)                                                     tGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATac                             <  2:913800/90‑1 (MQ=255)                                                      gCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATaca                            >  2:963056/1‑90 (MQ=255)                                                                 gATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTc                 <  1:885740/90‑1 (MQ=255)                                                                       ttCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCAtttt           >  1:827684/1‑90 (MQ=255)                                                                            ggCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTcagca      >  2:766974/1‑90 (MQ=255)                                                                             gCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTcagcag     <  2:827684/90‑1 (MQ=255)                                                                              cccAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACGCCACAACACTCCATTTTCAGCAgg    <  2:335583/90‑1 (MQ=255)                                                                                cAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGa                                              <  2:640247/46‑1 (MQ=255)                                                                                cAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGa                                              >  1:640247/1‑46 (MQ=255)                                                                                cAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGc  <  2:917620/90‑1 (MQ=255)                                                                                       |                                                                                   CCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGGC  >  NZ_CP009273/1547849‑1548017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑