| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 139,618 | A→G | E118G (GAA→GGA) | yadG → | ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 139,618 | 0 | A | G | 97.6% | 135.6 / ‑3.6 | 42 | E118G (GAA→GGA) | yadG | ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (24/17); total (24/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01 | |||||||||||
AACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTT > NZ_CP009273/139533‑139699 | aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg < 1:953044/90‑1 (MQ=255)aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAg < 1:81836/90‑1 (MQ=255) gTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCt > 1:495039/1‑90 (MQ=255) gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc > 2:56681/1‑90 (MQ=255) gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc > 2:562204/1‑90 (MQ=255) gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc > 1:996153/1‑90 (MQ=255) gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc > 1:640572/1‑90 (MQ=255) tGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCg < 2:836464/90‑1 (MQ=255) tGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCg < 1:255095/90‑1 (MQ=255) ttGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATggg > 2:791674/1‑90 (MQ=255) gAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACg > 1:672418/1‑90 (MQ=255) aggcaggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaacg > 2:478019/1‑90 (MQ=255) aggcaggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaacg > 1:884656/1‑90 (MQ=255) aggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaacgaacg < 1:307724/90‑1 (MQ=255) gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt > 1:144981/1‑90 (MQ=255) gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt < 1:722805/90‑1 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgcgc < 2:703505/84‑1 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgcgc > 1:703505/1‑84 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt > 2:767811/1‑90 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt > 2:758052/1‑90 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt > 2:280613/1‑90 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt > 2:419538/1‑90 (MQ=255) cGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGtt > 2:353068/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 1:739266/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 1:444401/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 1:384989/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 2:733501/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 2:73106/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 2:620669/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 2:608676/1‑90 (MQ=255) ggAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATc < 1:267543/90‑1 (MQ=255) ggAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATc < 2:384989/90‑1 (MQ=255) gcAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCg > 1:493840/1‑90 (MQ=255) cAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCgg < 1:353068/90‑1 (MQ=255) aagaagCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGa < 1:1892/90‑1 (MQ=255) aagCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGa < 2:183736/90‑1 (MQ=255) tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc < 1:277049/90‑1 (MQ=255) tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc < 1:56681/90‑1 (MQ=255) tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc < 2:897202/90‑1 (MQ=255) tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGCGCGCGTATGTTATCTGGCGGGATGAAgcgc < 1:119240/90‑1 (MQ=255) aCATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGcc < 2:884656/90‑1 (MQ=255) ccGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTTTCTGGCGGGATGAAGCGCCGttt < 2:770275/90‑1 (MQ=255) | AACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTT > NZ_CP009273/139533‑139699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |