Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,673,542	A→G	K287E (AAG→GAG)	ydgH →	DUF1471 family protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,673,542	0	A	G	100.0%	125.7 / NA	44	K287E (AAG→GAG)	ydgH	DUF1471 family protein YdgH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/29);  total (15/29)

ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAA  >  NZ_CP009273/1673458‑1673624                                                                                     |                                                                                   atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                               <  1:924736/90‑1 (MQ=255) atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                               <  1:750782/90‑1 (MQ=255) atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                               <  2:295629/90‑1 (MQ=255)  tgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAg                                                                              <  1:145271/90‑1 (MQ=255)   ggTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAgg                                                                             >  2:838004/1‑90 (MQ=255)   ggTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAgg                                                                             >  2:80924/1‑90 (MQ=255)     tCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTg                                                                           >  1:199625/1‑90 (MQ=255)       ccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt                                                                         <  1:792122/90‑1 (MQ=255)        cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa                                                                        >  2:926347/1‑90 (MQ=255)        cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa                                                                        <  1:838004/90‑1 (MQ=255)         gTTGGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                                       >  2:12049/1‑90 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                               <  1:365051/82‑1 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                                               >  2:365051/1‑82 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                                       >  2:425791/1‑90 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                                       <  2:151791/90‑1 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                                       <  2:836066/90‑1 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                                       <  1:952591/90‑1 (MQ=255)                   aTCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACcac                                                             <  1:236181/90‑1 (MQ=255)                   aTCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACcac                                                             <  1:80924/90‑1 (MQ=255)                               ggCACCTAGGACAACACGACACAAGTCTCCTATCAGGTAGCAAAAGGTGCCGCGGAGAAAGGTGTAAAGTATTCCCCCATCCCCCGCCAg                                                 <  1:875188/90‑1 (MQ=255)                               ggCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAg                                                 >  2:997772/1‑90 (MQ=255)                                           aaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGt                                     <  2:199625/90‑1 (MQ=255)                                           aaCATGACCGAAGTCTCCTATCAGGTTGCAAACCGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGt                                     <  2:133707/90‑1 (MQ=255)                                           aaCATGACCGAAGTCTCCTATAGGGTTGCAAACGGGGCGGGGGAAAAGGGTGCTAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGt                                     <  1:744086/90‑1 (MQ=255)                                              aTGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGt                                  >  2:684482/1‑90 (MQ=255)                                               tgcccGAAGTCTCCTATCAGGTTGCAAACCGTGCCGGGGAAAAAGGTGCTAATTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGta                                 <  1:684482/87‑1 (MQ=255)                                                 aCCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGtaat                               <  2:574251/90‑1 (MQ=255)                                                         ctcCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGAc                       >  1:136967/1‑90 (MQ=255)                                                           ccTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCg                     >  2:84599/1‑90 (MQ=255)                                                             taccAGGTTGCAAAACGTGCCGCGGAAAAAGGTGCTAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc                   <  2:613346/87‑1 (MQ=255)                                                             tATCAGTTTGCAAACCGTGCCGGGGAAAAAGGTGCTAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc                   <  1:327405/90‑1 (MQ=255)                                                             tATCAGGTTGCGAAACGTGCCGGGGAAAAGGGTGCAAAGTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc                   <  1:533618/90‑1 (MQ=255)                                                             tATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc                   <  2:136967/90‑1 (MQ=255)                                                             tATCAGGTTGCAAAACGTGCCGCGGAAAAAGGTGCTAAGTATTACCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc                   <  2:117831/90‑1 (MQ=255)                                                             tATCAGGTTGAGAAACGTGCCGGGGAAAAGGGTGCTAATTATTACCACACCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc                   <  1:539422/90‑1 (MQ=255)                                                                  ggTTGCGAACCGGGCCGGGGAGAAGGGTGCTAAGTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc              <  1:582210/90‑1 (MQ=255)                                                                  ggTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGGGGTAATAACCTGACCGTCAgcgc              >  1:898966/1‑90 (MQ=255)                                                                  ggTTGCAAACCGGGCCGGGGAAAAAGGTGCAAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc              <  1:84599/90‑1 (MQ=255)                                                                   gTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAg                                                 <  2:488754/54‑1 (MQ=255)                                                                   gTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAg                                                 >  1:488754/1‑54 (MQ=255)                                                                      gCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGAt          >  2:386990/1‑90 (MQ=255)                                                                       cGAAACGTGCCGCGGAGAAAGGTGCAAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc         <  2:34900/90‑1 (MQ=255)                                                                            cGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGtat    >  2:189372/1‑90 (MQ=255)                                                                              ggCCGCGGAGAAAGGTGTTAATTTTTCCCCCACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataa  <  1:919319/89‑1 (MQ=255)                                                                                     |                                                                                   ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAA  >  NZ_CP009273/1673458‑1673624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑