Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273497,0200AC54.2% 8.4 / 31.1 24*559E (TAA→GAA) ybaLKef family K(+) transporter
Reads supporting (aligned to +/- strand):  ref base A (11/0);  new base C (0/13);  total (11/13)
Fisher's exact test for biased strand distribution p-value = 4.01e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-05
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATT  >  NZ_CP009273/496936‑497107
                                                                                    |                                                                                       
ttGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCg                                                                                    >  2:626363/1‑90 (MQ=255)
                        gcgggCGCGTTTTACCATGCCTACTTTTTTTTCATATTTTACATCCGGCACCCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCg                                                            <  1:1782877/87‑1 (MQ=255)
                        gCTGGCGCTTCTTATCATCCCTACATTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCg                                                            <  2:304923/90‑1 (MQ=255)
                             cgcgTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTcc                                                       >  1:1011956/1‑90 (MQ=255)
                                ttCTTATCATCCCTAATTTTTTTTCATATTTTCCCCCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCcagc                                                    <  2:1525506/89‑1 (MQ=255)
                                gTCTTATCATGCCTCCATTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCcagc                                                    <  2:375585/90‑1 (MQ=255)
                                gTCTTATCATGCCTACATTTTTTTCATATTTTACATCCGGCAACCACCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCcagc                                                    <  1:674670/90‑1 (MQ=255)
                                acagtATCATGCCTACTTTTTTTTCATTTTTTCCACCGGACACCCCCCTTTCCCCCCCCCCCCCCCCCCCCCGCCGGTGGCGTTTCcagc                                                    <  2:1192526/86‑1 (MQ=255)
                                          gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa                                          >  1:464418/1‑90 (MQ=255)
                                           ccTACATTTTTTTCATTTTTTACATCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt                                         <  1:988004/90‑1 (MQ=255)
                                           ccTACATTTTTTTAATTTTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt                                         <  1:1307759/90‑1 (MQ=255)
                                           ccTACATTTTTTTAATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt                                         <  1:1455019/90‑1 (MQ=255)
                                           ccTACATTTTTTTAATTTTTTACACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt                                         <  1:1442509/90‑1 (MQ=255)
                                              aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCactgt                                      >  1:1192526/1‑86 (MQ=255)
                                              aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt                                      >  1:1579783/1‑90 (MQ=255)
                                                    ttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGc                                >  2:1696743/1‑90 (MQ=255)
                                                          tatTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc                          >  2:5294/1‑90 (MQ=255)
                                                                  acccGCCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc                  <  1:1684581/88‑1 (MQ=255)
                                                                   cccGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                 <  1:801346/89‑1 (MQ=255)
                                                                      ggCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt              >  2:1248649/1‑90 (MQ=255)
                                                                      ggCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt              >  1:463174/1‑90 (MQ=255)
                                                                             cccGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAcc       <  1:1350276/89‑1 (MQ=255)
                                                                                gTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGa    >  2:1871866/1‑90 (MQ=255)
                                                                                  ttACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGAtt  >  2:1674203/1‑90 (MQ=255)
                                                                                    |                                                                                       
TTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATT  >  NZ_CP009273/496936‑497107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑