| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,832,718 | 0 | T | G | 52.5% | 68.4 / 77.2 | 61 | intergenic (‑95/‑165) | ascG/ascF | DNA‑binding transcriptional regulator AscG/PTS cellobiose/arbutin/salicin transporter subunit IIBC |
| Reads supporting (aligned to +/- strand): ref base T (13/16); new base G (32/0); total (45/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.35e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-07 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATT > NZ_CP009273/2832636‑2832803 | tttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaa < 1:1622876/90‑1 (MQ=255)tttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGGGACCGGGTTTACaa > 2:1902126/1‑90 (MQ=255) cAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAATATTTAGGAGACCCGGTTCACAAATATaaaa > 1:1172901/1‑90 (MQ=255) aCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGaa < 2:1377208/90‑1 (MQ=255) aCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTTAGGGGACCGGGTTCACAAATATAAAAAATGaa > 1:172932/1‑90 (MQ=255) cGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAc < 1:1035019/90‑1 (MQ=255) aGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTTAGGGGACCGGGTTCACAAATATAAAAAATGAACAAt > 2:1532612/1‑90 (MQ=255) aGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAAt > 2:1838056/1‑90 (MQ=255) aGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTTAGGTGACCGGGGTCACAAATATAAAAAATGAACAAt > 1:1176459/1‑90 (MQ=255) aGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTCAGGGGACCGGTGTCACAAATATAAAAAAAGAACAAt > 1:1238332/1‑90 (MQ=255) gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAtt < 1:1237116/90‑1 (MQ=255) tgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTTAGGTGACCGGGTTCACAAATATAAAAAATGAAGAATTc > 2:106036/1‑90 (MQ=255) tgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGGGACCGGTTTCACAAATATAAAAAATGAACAATTc > 2:1664362/1‑90 (MQ=255) tACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCAc < 1:1454280/90‑1 (MQ=255) tACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCAc > 2:249358/1‑90 (MQ=255) cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActc < 2:1330838/90‑1 (MQ=255) cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTCCGGGGACCGGGTGCACAAAAATAAAAAATGGACAAATCActc > 1:1250450/1‑90 (MQ=255) tGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGGGACCGGGTTCACAAATATAAAAAATGAACAATTCActct > 2:1588091/1‑90 (MQ=255) gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAATATTTAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTTActctc > 1:1970737/1‑90 (MQ=255) gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActctc > 2:489317/1‑90 (MQ=255) gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActctc < 2:2015327/90‑1 (MQ=255) gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCActctc > 2:690951/1‑90 (MQ=255) gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCActctc > 2:458821/1‑90 (MQ=255) gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAAAGAACAATTCActctc > 1:527280/1‑90 (MQ=255) gCACCGTTTGCCAATTGTCCTTGCACAATCGGCGGGGAAAAAATTTAGGGGGCCGGGTTCACAAAAATAAAAAAAGAACAAATTActctc > 1:1868313/1‑90 (MQ=255) ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAAAATTCAGGTGACCGGGTTTACAAAAATAAAAAAAGAAAAATTCACTCTCTTg > 1:1633992/1‑90 (MQ=255) ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTg > 2:179590/1‑90 (MQ=255) ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGGGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCttt > 2:1594905/1‑89 (MQ=255) ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTGTGGGGACCGGGGGCCCAAATATAAAAAATGAAGCATTCACTCTCTTg > 1:909034/1‑90 (MQ=255) ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTCAGGTGACCGGGGTCACAAATATAAAAAATGAACAATTCACTCTCTTg > 1:1682314/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAATATTTAGGGGACCGGGTTCACAAAAATAAAAAATGAACAATTCACTCTCTTGCt > 1:1928227/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAATATTCAGGTGGCCGGTTTCACAAATATAAAAAAAGAACAATTCAATCTCTTggt > 2:1859870/1‑88 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAAAATTCAGGTGACCGGGGTCACAAATATAAAAAAAGAACAATTCACTCTCTTGCt > 1:1769328/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTTAGGGGACCGGTTTCACAAATATAAAAAAAGAACAATTTACTCTCTTGCt > 2:1188005/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCAc > 1:644087/1‑81 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt > 1:758283/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt > 2:1293297/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt > 2:449524/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt > 2:67134/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATAATTAGGGGGCCGGGGTCCCAAATAAAAAAAAATTACAAATCACTTTCTTGCt > 1:1273466/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTTAGGTGACCGGGTTCACAAATATAAAAAAAGAACAATTCACTCTCTTTCt > 1:1594251/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTCAGGGGGCCGGGTTCACAAATATAAAAAAAGAACAAATCACTCTCTTGCt > 1:1506455/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTCAGGGGACCGGGTTCACAAAAATAAAAAAAAAACAATTCACTCTCTTggt > 2:882488/1‑88 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCCGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCAc < 2:644087/81‑1 (MQ=255) gTTTGCCGATTGTCCTTGCAAAATCGGCGGGAAAAATATTCAGGTGGCCGGGGCGGCAAATATAAAAAATTAACAATTTTCTCTCTTggt > 2:1205371/1‑88 (MQ=255) tGCCGATTGTCCTTGCACAATCGGCGGGGAAAATATTCAGGAGACCGGGTTCACAAAAATAAAAAATGAACAAATCAACCTCCTCCTTAt > 2:1391674/1‑90 (MQ=255) gATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAg < 1:1589068/90‑1 (MQ=255) cTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc < 1:306545/90‑1 (MQ=255) cTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc < 1:1902126/90‑1 (MQ=255) ttGCACAATCGGCGGGAAAAATATTCAGGGGACCGGGTTCACAAATATAAAAAAAGAACAATTCACTCTCTTGCTTTTTTTGTGACAACt > 1:1122131/1‑90 (MQ=255) tGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTa < 2:1633992/90‑1 (MQ=255) aaTCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTg < 2:1646953/78‑1 (MQ=255) aaTCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTg > 1:1646953/1‑78 (MQ=255) aTCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATg > 2:824696/1‑90 (MQ=255) aTCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATg > 2:1680544/1‑90 (MQ=255) aTCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAAAAATTCACTCTCTTGCTTATTTTGTGACAAATATTCATg > 1:172373/1‑90 (MQ=255) aTCGGCGGGAAAAATATTCAGGGGGCCGGGTTCACAAATATAAAAAAAGAAAAATTTACTTTCTTGCTTATTTAATGACAAATATTTATg > 1:1882621/1‑90 (MQ=255) ttCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCCCTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGt < 2:2003535/90‑1 (MQ=255) ttCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGt < 1:1293297/90‑1 (MQ=255) ccGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAtt > 2:463341/1‑90 (MQ=255) ccGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAtt < 2:172932/90‑1 (MQ=255) | TTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATT > NZ_CP009273/2832636‑2832803 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |