Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,781,3850TG53.7% 79.1 / 100.6 67G396G (GGT→GGGenvCmurein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base T (8/23);  new base G (36/0);  total (44/23)
Fisher's exact test for biased strand distribution p-value = 1.49e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-11
Rejected as consensus: Frequency below/above cutoff threshold.

GCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGTTTTG  >  NZ_CP009273/3781297‑3781463
                                                                                        |                                                                              
gCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTc                                                                               <  2:389124/90‑1 (MQ=255)
 cTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCg                                                                              <  1:1380328/90‑1 (MQ=255)
 cTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCg                                                                              >  2:298844/1‑90 (MQ=255)
 cTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCg                                                                              >  2:1118953/1‑90 (MQ=255)
         agagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCAc                                                                      <  1:2057793/90‑1 (MQ=255)
          gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCCGGGGGGGCCCTCAct                                                                     >  1:785993/1‑90 (MQ=255)
          gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGGCTTCCct                                                                     >  1:644749/1‑90 (MQ=255)
          gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGGCCTCAct                                                                     >  2:1498532/1‑90 (MQ=255)
          gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCAct                                                                     >  2:802223/1‑90 (MQ=255)
          gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCAct                                                                     >  2:66028/1‑90 (MQ=255)
          gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCAct                                                                     >  2:656533/1‑90 (MQ=255)
          gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCAct                                                                     >  2:1607890/1‑90 (MQ=255)
          gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGGGGCCTTCAcc                                                                     >  1:965225/1‑89 (MQ=255)
          gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGCGGCCTTCAct                                                                     >  1:1992215/1‑90 (MQ=255)
               cACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTAtt                                                                >  2:1604937/1‑90 (MQ=255)
                    gTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCCTCACCCTATTTCGaa                                                           >  1:621021/1‑90 (MQ=255)
                      gAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCActc                                                                    >  2:1383778/1‑79 (MQ=255)
                      gAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCActc                                                                    <  1:1383778/79‑1 (MQ=255)
                      gAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAAt                                                         >  2:1183094/1‑90 (MQ=255)
                         cGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTcg                                                      >  2:220653/1‑90 (MQ=255)
                          gTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTcgc                                                     <  2:57618/90‑1 (MQ=255)
                           ttGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTAGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTcgcc                                                    >  1:23403/1‑90 (MQ=255)
                             ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTTGAAAATcgccgc                                                  >  1:1178106/1‑90 (MQ=255)
                             ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTTGAAATTcgccgc                                                  >  2:2040486/1‑90 (MQ=255)
                             ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTTGAAATTcgccgc                                                  >  1:713354/1‑90 (MQ=255)
                             ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTcgccgc                                                  >  2:742566/1‑90 (MQ=255)
                             ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCCTCCCCCTATTTTGGAAATcgccgc                                                  >  1:435519/1‑90 (MQ=255)
                             ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGGGGCCTTCACTCTATTTCGAAATTtgccgc                                                  >  1:1667229/1‑90 (MQ=255)
                             ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGCGGGCTTCACTCTATTTTGAAATTtgccgc                                                  >  2:1536272/1‑90 (MQ=255)
                             ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGCGGGCCTCCCCCTCTTTCGGAATTtgccgc                                                  >  1:1205227/1‑90 (MQ=255)
                             ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGCGGCCTTCCCTCTATTTTGAAATTcgccgc                                                  >  1:325130/1‑90 (MQ=255)
                             ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGCGGCCTTCCCTCTATTTCGGAATTcgccgc                                                  >  1:1814066/1‑90 (MQ=255)
                             ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGCGGCCTTCACTCTATTTTGAAATTcgccgc                                                  >  1:1490737/1‑90 (MQ=255)
                                  gCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTCGCCGCCCggg                                             >  2:1905258/1‑90 (MQ=255)
                                       ttCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAgg                                        <  1:1649800/90‑1 (MQ=255)
                                        tCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGcc                                                                           >  2:1796794/1‑54 (MQ=255)
                                        tCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGcc                                                                           <  1:1796794/54‑1 (MQ=255)
                                         cgcgcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCg                                      <  1:1943015/90‑1 (MQ=255)
                                            gcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                   <  1:27946/90‑1 (MQ=255)
                                            gcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                   <  2:1205227/90‑1 (MQ=255)
                                            gcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                   <  2:1756090/90‑1 (MQ=255)
                                            gcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                   <  2:1575246/90‑1 (MQ=255)
                                            gcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                   <  1:438418/90‑1 (MQ=255)
                                            gcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                   <  2:1526048/90‑1 (MQ=255)
                                            gcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCAGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                   <  1:404643/90‑1 (MQ=255)
                                              gggCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCaa                                 <  2:1472715/90‑1 (MQ=255)
                                                 ccAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATcc                              <  2:1479968/90‑1 (MQ=255)
                                                   aGCCGATTGCACTGGTGGGCAGCAGTGGGGGTCAGGGTCGGCCTTCCCTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCcac                            <  2:170793/90‑1 (MQ=255)
                                                         ttGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTc                                                       >  1:507663/1‑57 (MQ=255)
                                                         ttGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTc                                                       <  2:507663/57‑1 (MQ=255)
                                                         ttGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTTGAAATTCGCCGCCCGGGGCAGGCGGGCAATCCCCAGCCGt                      >  1:492880/1‑90 (MQ=255)
                                                              cTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGGAAATCTCCGCCAGGGTCCGGCGGTCAATCCCCATCCGTGGGTg                 >  2:1093026/1‑90 (MQ=255)
                                                                ggtggGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTTGCCGCCAGGGGCAGGCGGGCAATCCACAGCCGGGGGTggg               >  2:1206356/1‑90 (MQ=255)
                                                                 gtggGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGa              <  1:80361/90‑1 (MQ=255)
                                                                     gcagcaGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGGCAGGCGGGCAATCCCCAGCCGGGGGTGGGGAGAt          >  1:138573/1‑90 (MQ=255)
                                                                      cagcagTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGGCAGGGGGGCAATCCACAGCCGGGGTTGGGGAGATa         >  1:1614083/1‑90 (MQ=255)
                                                                          agTGGCGGTCAGGGTCGGGCTTCCCTCTATTTTGGAATTCGCCGCCAGGGTCAGGCGGTGAATCCACAGGCCGGGTTGGGGAGATAAGtt     >  2:508684/1‑90 (MQ=255)
                                                                          agTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTcgc                                                     <  2:1686764/42‑1 (MQ=255)
                                                                          agTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                   <  1:1896151/60‑1 (MQ=255)
                                                                          agTGGCGGTCAGGGTCGGCCCTCACTCTCTTTCGAAATTCGCCGCCAGGGGCAGGCGGGCAAACCCCAGCCGGGGTTGGGGAGATAAGtt     >  1:1015330/1‑90 (MQ=255)
                                                                          agTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTcgc                                                     >  1:1686764/1‑42 (MQ=255)
                                                                          agTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                   >  2:1896151/1‑60 (MQ=255)
                                                                          agTGGCGGGCAGGGGCGGCCTTCACTCTATTTTGAAAATTGCCGCCCGGGGCAGGGGGGGAATCCACAGCCGGGGGTGGGGAGAAAAGtt     >  1:1430863/1‑90 (MQ=255)
                                                                             ggCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                   <  1:1316407/57‑1 (MQ=255)
                                                                             ggCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                   >  2:1316407/1‑57 (MQ=255)
                                                                             ggCGGTCAGGGGCGGCCTTCACTCTATTTTGAAAATCGCCGCCCGGGGCAGGCGGGCAAACCACAGCCGTGGGTGGGGAGATAAGGTTTg  >  1:1508026/1‑90 (MQ=255)
                                                                             ggCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTTGCCGCCCGGGGCAGGGGGGCAATCCCCAGCCCTGGGTGGGGAGATAAGTTTTg  >  1:1980933/1‑90 (MQ=255)
                                                                                        |                                                                              
GCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGTTTTG  >  NZ_CP009273/3781297‑3781463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: