| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 687,303 | 0 | A | G | 48.6% | 74.9 / 29.9 | 74 | intergenic (‑63/+27) | corC/ybeY | CNNM family magnesium/cobalt transport protein CorC/rRNA maturation RNase YbeY |
| Reads supporting (aligned to +/- strand): ref base A (8/30); new base G (36/0); total (44/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTT > NZ_CP009273/687214‑687388 | actactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGa > 1:117741/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGTCTg > 1:666601/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGTCTg > 1:136090/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 1:836181/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 1:390085/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:364280/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:1753577/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:25865/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 1:1409592/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:183887/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 1:2056015/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:23876/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCtt > 2:1975261/1‑89 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 1:775034/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 2:1917923/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 2:1291647/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACggtcg > 1:1238464/1‑86 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTg > 1:1877407/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTg > 1:2071020/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTg > 2:254238/1‑90 (MQ=255) tgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGc < 2:2008735/90‑1 (MQ=255) gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGcc > 1:1495690/1‑90 (MQ=255) gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGcc > 2:2060858/1‑90 (MQ=255) gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGcc < 2:1877407/90‑1 (MQ=255) aaTTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGTTGCCGGt > 1:288192/1‑90 (MQ=255) gtcgtcGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCaaa > 1:1048762/1‑90 (MQ=255) gtcgCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTGTGCCCATCAAAAGc > 2:1581451/1‑90 (MQ=255) cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTgg < 2:265669/90‑1 (MQ=255) aTGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGTCAAAAGCCTGGCaa > 2:643741/1‑90 (MQ=255) aTGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGTCAAAAGCCTGGCaa > 2:1247771/1‑90 (MQ=255) aTGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACg < 1:1756415/67‑1 (MQ=255) aTGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACg > 2:1756415/1‑67 (MQ=255) tGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCaaa < 2:1012120/90‑1 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCAGTCAAATGCCTGGCAAattatt > 2:483974/1‑90 (MQ=255) cgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGTCAAAAGCCTGGCAAaatattt > 2:1991506/1‑89 (MQ=255) tGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctttc < 1:254238/90‑1 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCCCCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc < 2:956244/90‑1 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGTCTGCCCGTCAAAAGCCCGGCAAATTATTTTTTTTCGGc > 1:44254/1‑90 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGcc > 1:662144/1‑57 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGACAAAAACCTGGCAAATTATTCTTTTTCGGc > 1:1492507/1‑90 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGcc < 2:662144/57‑1 (MQ=255) ctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAGCCTGGCAAATTATTTTTTTTCCGCa > 2:1276043/1‑90 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttct < 1:553877/80‑1 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttct > 2:553877/1‑80 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCaa < 2:778421/90‑1 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCaa < 1:1749283/90‑1 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCaa < 2:1394274/90‑1 (MQ=255) tGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGt < 1:1326465/90‑1 (MQ=255) gttagttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGGCAAAAACCTGGCAAATTATTTTTTTTCCGCCATGTa > 2:2000559/1‑90 (MQ=255) ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTAc < 2:117741/90‑1 (MQ=255) agttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACgg < 1:25865/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 1:1581451/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 2:740274/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 2:390085/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 2:2071020/90‑1 (MQ=255) aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGc > 1:446355/1‑43 (MQ=255) aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCAAAAGACTGGCAAATTATTTTTTTTCCGCAATGTACGGATcc > 1:1259853/1‑90 (MQ=255) aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCAAAAACCTGGCAAAATATTTTTTTTCGGCAATGTACGGGTcc > 1:137515/1‑90 (MQ=255) aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAACCTGGGAAATTTTTTTTTCTCGGCAATGTACGGATcc > 1:2046469/1‑90 (MQ=255) aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAACCCGGCCAATTATTTTTTTTCGGCAATGTACGGATcc > 1:1044156/1‑90 (MQ=255) aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGc < 2:446355/43‑1 (MQ=255) aTCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCaa > 2:1339254/1‑60 (MQ=255) aTCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCaa < 1:1339254/60‑1 (MQ=255) aaaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCAt < 2:180508/90‑1 (MQ=255) gCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc < 2:3635/90‑1 (MQ=255) gCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc < 2:2046469/90‑1 (MQ=255) gtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagca < 1:879874/90‑1 (MQ=255) ttAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaa < 1:2070407/90‑1 (MQ=255) ttAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaa < 1:407750/90‑1 (MQ=255) tCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCAt < 2:472491/90‑1 (MQ=255) cACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATa < 2:1951358/90‑1 (MQ=255) aaCGGCGGGGGCGGCTGCCCGTCAAAAGCCTGGCAAAATATTTTTTTTCGGCAATGTACGGGTCCTCATAACCCCGAGCAAGCATAAtct > 1:1563776/1‑90 (MQ=255) aaCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAAtct < 2:1495690/90‑1 (MQ=255) aCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAAtctc > 2:343352/1‑90 (MQ=255) ggggGCGTCTGCCAGTAAAAAGCCTGGCAAATTATTTTTTTTCGGCAATGTACGGATCCTCATAACCCAGAGCAAGCATAATCTCTGttt > 1:1558289/1‑90 (MQ=255) | ACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTT > NZ_CP009273/687214‑687388 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |