| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,410 | 0 | A | T | 48.4% | 30.6 / 14.8 | 31 | I100N (ATT→AAT) | ybhS | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (14/2); new base T (0/15); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.09e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACC‑AGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTG > NZ_CP009273/822330‑822495 | tGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACC‑AGACCGCGAATTTTCCCCg > 2:1719519/1‑90 (MQ=255) aTCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACC‑AGACCGCGAATTTTCCCCGCCTGCATTTTg > 1:121692/1‑90 (MQ=255) tgcggtgGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACC‑AGACCGCGAATTTTCCCCGCCTGCATTTTGGCGAt > 2:2023114/1‑90 (MQ=255) ggggtgGCGTGGGCGCCCCCCATCTGTCCCGCAAAACCCCCCGGAATAACCCCC‑AGCCCGCGATTTTTCCCCGCCTGCATTTTGGCGATc < 2:1216272/88‑1 (MQ=255) gcggggGCTTGGGCCCGCCCCTTCTTTCCCCAAAAACCCCCCGAAATACCCCCC‑AGCCCGCAATTTTCCCCCGCCTGCATTTTGGCGATc < 1:211699/90‑1 (MQ=255) gcggggGCGTGGGCGCCCTCCTTCTGTCCCGAAAAACCCCCCGAAAAACCCCCC‑AGCCCGCGATTTTCCCCCGCCTGCATTTTGGCGATc < 1:2082593/90‑1 (MQ=255) gcggggGCGTGGGCCCCCTCCATCTGTCCCGCAAAACCCCCCGAAATACCCCCC‑AGACCGCGATTTTCCCCCGCCTGCATTTTGGCGATc < 1:77348/90‑1 (MQ=255) gtgggCGCGCCCCATCTGTCCCGAAAAACCCCCCGAAAAACCCCCC‑AGCCCGCGATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTg < 2:1817319/87‑1 (MQ=255) gcccTCCATCTGTCCCCCAAAACCCCCCGAAATACCCCCC‑AGCCCGCGATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGtt < 1:137694/87‑1 (MQ=255) cgcTCCTTTTGTCCCGAAAAACCCCCCGGAATACCCCCC‑AGCCCGCAATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTa < 2:1343949/90‑1 (MQ=255) gcTCCATCTGTTCCGCAAAATCCACCGGAATAACCACC‑AGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTAt > 2:816904/1‑90 (MQ=255) gcTCCATCTGTTCCGCAAAATCCACCGGAATAACCACC‑AGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTAt > 1:479045/1‑90 (MQ=255) aTCTGTTCCGCAAAATCCACCGGAATAACCACC‑AGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTg > 2:1694364/1‑90 (MQ=255) aTCTGTTCCGCAAAATCCACCGGAATAACCACC‑AGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTg > 2:816100/1‑90 (MQ=255) gCAAAATCCACCGGAATAACCACC‑AGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCg > 1:56064/1‑90 (MQ=255) aaaaaCCCCCCGAAAAACCCCCC‑AGCCCGC‑AATTTTTCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTc < 1:151686/89‑1 (MQ=255) aaaaCCCACCGGAATACCCCCC‑AGACCGCGATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTc < 2:56064/90‑1 (MQ=255) aaaaCCCCCGGAAAAACCCCCAAGCCCGCAATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTc < 2:2052829/90‑1 (MQ=255) aTCCACCGGAATAACCACC‑AGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGAt > 2:930322/1‑90 (MQ=255) aaaaCCACC‑AGACCGCGATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGaa < 2:1701886/88‑1 (MQ=255) aTACCCCCC‑AGCCCGCGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGaa < 2:401071/90‑1 (MQ=255) aaCCCCC‑AGCCCGGGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAAcc < 1:50413/90‑1 (MQ=255) caaaCCGCGATTTCCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCAt < 1:1376388/87‑1 (MQ=255) aCCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGgt > 1:2081024/1‑90 (MQ=255) ccGCGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTc < 1:1047391/84‑1 (MQ=255) ccGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTc > 2:1047391/1‑84 (MQ=255) ccGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGgtg > 1:220646/1‑90 (MQ=255) cgcaAATTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGgtgt < 2:352131/90‑1 (MQ=255) gcgATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGtgtg < 1:1560199/90‑1 (MQ=255) gcgAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGtgtg > 1:807755/1‑90 (MQ=255) gcgAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGtgtg > 2:418473/1‑90 (MQ=255) | TGATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACC‑AGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTG > NZ_CP009273/822330‑822495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |