| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,074,924 | 0 | A | G | 52.2% | 17.9 / 30.4 | 23 | D55G (GAT→GGT) | putP | sodium/proline symporter PutP |
| Reads supporting (aligned to +/- strand): ref base A (0/11); new base G (12/0); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.40e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.04e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGC‑GGCTGGCT‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTG > NZ_CP009273/1074837‑1075006 | ggCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGata < 2:1614200/90‑1 (MQ=255) tGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGC‑GGCTGGCT‑GTTAAt < 1:1293231/90‑1 (MQ=255) gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGGGGGGGGTATTAGC‑GGGTGGGT‑GGTTATTGGGGTGCCGGgggc > 2:1138575/1‑90 (MQ=255) gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTGTGGGG‑GGGGGGGT‑GGTGATGGGGGTGGCgggggg > 1:999745/1‑87 (MQ=255) gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGG‑GGGTGGGTGGTTATTGGGGTGGCgggggc > 2:1250857/1‑88 (MQ=255) gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGC‑GGGGGGGG‑GGTTATGGGGGTGGCgggggg > 2:773293/1‑87 (MQ=255) acaggTCTTGGGCCATTCGTGGCGGCATTATCGGCGGGTGCGTCGGATATGAGC‑GGCTGGCT‑GTTAATGGGGTTGCCGGGCGCT‑G‑ttttt < 2:1834899/86‑1 (MQ=255) aGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGGGC‑GGGTGGGT‑GTTTATGGGGGTTGCGGGCGGC‑G‑tttttttt > 2:646750/1‑90 (MQ=255) tGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATGAGG‑GGCTGGCT‑GTTTATGGGGGTGGCGGGGGCG‑G‑Ttttttttttccg > 2:186146/1‑86 (MQ=255) tGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATTAGC‑GGGGGGGT‑GGTTATTGGGGTGGCGGGGGGG‑G‑Ttttttttttttg > 2:909329/1‑86 (MQ=255) tGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGCGGGGGGGGG‑GTTATTGGGGTGGCCGGGGGGG‑G‑GTtttttttcgt > 2:1110626/1‑88 (MQ=255) tGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGGATTAGG‑GGGTGGGT‑GTTTATGGGGGTGGCGGGGGCG‑G‑TTTTTTTTTTCgg > 2:1424549/1‑90 (MQ=255) tGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTGTGCTG‑GGCTGGCT‑GGTTATGGGGGTGTTCGGCGCT‑G‑TGtttttttttgg > 2:58505/1‑86 (MQ=255) aTTCGTGACGGCATTATCGGCGGGTGCGTCGGGTATGAGG‑GGCGGGGT‑GTTTATGGGGGTGGCGGGGGCT‑GTTTTTTTTTTCCGGGtttt > 2:101079/1‑90 (MQ=255) aTTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGG‑GGGCGGCT‑GTTTATGGGGGTGGCGGGGGCTGG‑TTTTTTTTTCTGGGGttt > 2:2054387/1‑90 (MQ=255) tCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGC‑GGCTGGCT‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCg < 2:252115/90‑1 (MQ=255) cGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGC‑GGCTGGCT‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGa < 2:571961/90‑1 (MQ=255) ggcggGTGCGTCGGATATGAGC‑GGCTGGCT‑GTTTATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCAt < 1:147557/90‑1 (MQ=255) gcggGTGCGTCGGATATGAGC‑GGCTGGCT‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCAtt < 2:1798399/90‑1 (MQ=255) gTGCGTCGGATATGAGC‑GGCTGGCT‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGcc < 1:1250857/90‑1 (MQ=255) gTGCGTCGGATATGAGC‑GGCTGGCT‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGcc < 2:1863675/90‑1 (MQ=255) gTGCGTCGGATATGAGC‑GGCTGGCT‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGcc < 2:803062/90‑1 (MQ=255) gCGTCGGATATGAGC‑GGCTGGCT‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTg < 2:2084094/90‑1 (MQ=255) gCGTCGGATATGAGC‑GGCTGGCT‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTg < 2:178524/90‑1 (MQ=255) | GGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGC‑GGCTGGCT‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTG > NZ_CP009273/1074837‑1075006 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |