| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,955 | 0 | T | G | 55.3% | 35.4 / 49.1 | 38 | T495P (ACC→CCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (4/13); new base G (21/0); total (25/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCT > NZ_CP009273/822871‑823038 | cTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGgggggggg > 2:580172/1‑90 (MQ=255)cTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAggg > 2:543319/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAAC‑GCCGGGAg > 2:299759/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGTGGGGGCTAC‑GCCGGGAg > 1:451737/1‑90 (MQ=255) tggtgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAA‑ACCGGAAGGCg > 2:696750/1‑90 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAC‑GCCGGAAGTCGGTtc < 1:125850/90‑1 (MQ=255) cATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAC‑GCCGGGAGGCGGgtcgtc > 1:508803/1‑90 (MQ=255) tCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCga < 1:53615/90‑1 (MQ=255) ccGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGGACGGCCGGAAG‑CGGGTTGTCgagga > 1:1042420/1‑88 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCGGAAGTCGGGTCGTCGAGaaaa > 2:53615/1‑89 (MQ=255) aCGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGa < 2:1150490/90‑1 (MQ=255) tttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCGGGAGGCGGGTCGTCGGGAAACAGAAAGTc > 2:144076/1‑90 (MQ=255) tttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCCGAAGGCGGGTCGTCGAGAAAAAGaaaggc > 1:990402/1‑86 (MQ=255) aCCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAC‑GCCGGAAGGCGGGTCGTCGAGAAAAAGAATGTCCGGGTCa > 2:63797/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 2:269101/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAA‑GCCGGAAGGCGGGTTGTCGGGAAAAAAAAAGTCCGGGTCATGc > 2:1109342/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCGGGAGTCGGGTTGTCGGGAAAAAGAAAGTCCGGGTCATGc > 2:781360/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCGGGAGGCGGGTCGTCGAGAAAAAAAAAGGCCGGGTCATGc > 1:1163/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCGGAAGTCGGTTCGTCGGGAAAAAGAATGTCCGGGTCCTgg > 1:750442/1‑89 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCGGAAGGCGGGTCGTCGAGAAAAAGAATGTCCGGGTCATGc > 2:83312/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCCGGAGTCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGc > 1:205245/1‑90 (MQ=255) gCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCGGAAGTCGGGTCGTCGAGAAACAGAATGTCCGGGTCATGCAt > 2:228081/1‑90 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATc < 2:897853/90‑1 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg < 1:543319/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 1:781360/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 2:750442/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAA‑GCCGGAAGTCGGGTCGTCGCGAAAAAGAAAGTCCGGGTTCTGCATCAGCGaaa > 2:822987/1‑89 (MQ=255) gCAGCCAAAATTCACGGCGGGTGAGGGGGGCAAC‑GCCGGAAGTCGGTTCGTCGAGAAAAAGAATGTCCGGTTCATGCATCAGCGAACAgg > 1:284489/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGTGAGGGGGGCAAC‑GCCGGAAGTCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGAACAgg > 1:320062/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGGGAC‑CCCCCAAGGCGGTTCGCTGAGAAACAAAATATCCGGGTCATGGATCAGCGCACAgg > 1:1140847/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCGGTAGGCGGGTCGGCGTAAAACAGAAAAGGCGGGTTATGCAGCAGCGGACaag > 1:897853/1‑88 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCCGAAGTCGGTTTGTCGAGAAACAGAATGTCCGGGTTCTTCATCAGCGCACAgg > 2:911656/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgcc < 1:918416/90‑1 (MQ=255) aGCCAAAATTCACGGCGGGGGGGGGGGGGAAC‑GCCGGGAGTCGGGTCGTCGGGAAAAAGAAAGTGCGGGGCATGCCTCAGCGGAAAGgcc > 1:931361/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGGGGGGGGGGCAAC‑GCCGGAAGTCGGGTCGTCGAGAAAAAGAATGTCCGGGTTATGCATCAGCGAACAGgcc > 2:1042041/1‑90 (MQ=255) cggcggGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:940332/90‑1 (MQ=255) cggcggGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:83312/90‑1 (MQ=255) cggcggGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:1042987/90‑1 (MQ=255) ggcggGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCt < 2:725932/90‑1 (MQ=255) | CTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAC‑GCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCT > NZ_CP009273/822871‑823038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |