| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 970,379 | 0 | C | T | 58.3% | 10.4 / 25.5 | 24 | S202F (TCC→TTC) | mukF | chromosome partition protein MukF |
| Reads supporting (aligned to +/- strand): ref base C (1/9); new base T (14/0); total (15/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.65e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGT‑GAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGC > NZ_CP009273/970291‑970462 | cTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTcc < 1:727763/90‑1 (MQ=255) aCGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGGGGTTTTCCGCtgt‑gg > 2:1176595/1‑89 (MQ=255) aCGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGGGGGTTTCCGCtgt‑gg > 2:631390/1‑89 (MQ=255) aCGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGGGGGGGTTTTCCGCTGG‑GGAATGTTGGttt > 1:232690/1‑90 (MQ=255) cGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGGGATTTTCAGCTGG‑GAAATGTTGCtttt > 2:615699/1‑89 (MQ=255) cagcagcagcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGT‑GGATTGTTGCTTTCTGaa > 2:1028409/1‑90 (MQ=255) cagcagcagcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGT‑GAATTGTTGCTTTCGGaa < 1:1019679/90‑1 (MQ=255) cagcagcagcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGT‑GAATTGTTGCTTTCGGaa < 2:823224/90‑1 (MQ=255) agcagcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGGGGGGGTTTTCAGCTGTGGATTTTTTGTTTTTGGaaatt > 1:318083/1‑88 (MQ=255) gcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGGTTTTCCGCTGT‑GAATTGTTGCTTTTGGAAAATTcctg > 2:904673/1‑88 (MQ=255) gcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTTCAGCTGT‑GAATTGTTGCTTTTGGAAAAtttccg > 2:153586/1‑86 (MQ=255) aaGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGT‑GAATTGTTGCTTTCGGAAACTTCCGGAACGCTg < 2:318083/90‑1 (MQ=255) aGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGGTTTTCAGCTGT‑GGATTGTTGCTTTCGGAAACTTCCGGGAcgccgc > 1:186637/1‑90 (MQ=255) aGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGGTTTTCAGCTGT‑GAATTGTTTCTTTTTGAAACTTCCCGGACGCTGc > 2:858996/1‑90 (MQ=255) gATATCGCCCAGTTGCTGAACAAAGACTGGCGGGGGGGGGTTTTCAGCCGT‑GAATTTTTTGTTTTTGAAAATTCCCGAACGCcgcgggga > 1:249355/1‑86 (MQ=255) tCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGT‑GAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGc < 2:186637/90‑1 (MQ=255) gCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTTCAGCTGT‑GAATTTTTGCTTTTGGAAACTTCCGGAAAGCTGCGGGAATTTCAg > 2:1215184/1‑90 (MQ=255) cAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGT‑GAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGAt < 2:650333/90‑1 (MQ=255) gTTGCTGAACAAAGACTGGCGGGGGGCGGTTTTCAGCTGT‑GAATTTTTGGTTTTTGAAACTTCCGGGACGCGGCGTGGATTGGAGGATaa > 1:903382/1‑89 (MQ=255) aaaGACTGGCGGGCGGCGATTTTCCGCTGT‑GAATTTTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTTCAGGATAAGCTGGGAGCg > 2:725203/1‑90 (MQ=255) tGGCGGGCGGCGATTTTCCGCTGG‑GGATTTTTTCTTTTTGAAAAATCCGGGACGCCGCCGGAATTTGAGGGTACGCCGGGAGGGGCgggg > 1:556856/1‑89 (MQ=255) ggcggcGATTTCCAGCTGT‑GAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACaa < 1:416798/90‑1 (MQ=255) ggcggcGATTTCCAGCTGT‑GAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACaa < 1:1083935/90‑1 (MQ=255) cgATTTCCAGCTGT‑GAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGc < 1:858996/90‑1 (MQ=255) cgATTTCCAGCTGT‑GAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGc < 2:46080/90‑1 (MQ=255) | CTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGT‑GAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGCGACAAATTGC > NZ_CP009273/970291‑970462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |