| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,074,943 | 0 | A | T | 53.3% | 9.8 / 21.0 | 15 | L61F (TTA→TTT) | putP | sodium/proline symporter PutP |
| Reads supporting (aligned to +/- strand): ref base A (1/6); new base T (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTG‑TTAAT‑GGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGA > NZ_CP009273/1074871‑1075022 | gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTG‑TTAAT‑GGGGTTGCCGGgcgc < 2:164781/90‑1 (MQ=255)gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGTGGGTATTAGCGGGGTGCTG‑TTTAT‑TGGGGTGCCgggggg > 2:1033428/1‑87 (MQ=255)gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGGGGGGGGCTG‑TTTAT‑TGGGGTGGCgggggg > 2:109420/1‑87 (MQ=255) tGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGGATGAGGGGGGGGCTG‑TTTAT‑GGGGGGGGCGGGGGGCGTTTTTTTTTTCgg > 2:825037/1‑90 (MQ=255) tGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGGATGAGGGGCGGGGGGTTTTTT‑GGGGTGGCGGGGGGGGGTTtttttttctg > 2:903904/1‑88 (MQ=255) aTTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGGGGGTGGGTG‑TTTATGGGGGTGGCGGGGGGCGTTTTTTTTTTCCGGGtttt > 2:1183764/1‑90 (MQ=255) aTTCGTGACGGCATTATCGGCGGGGGCGTCGGATATGAGCGGCTGGCTG‑TTTAT‑GGGGGTGCCGGGGGCTGTTTTTTTTTTCGGGGtttt > 2:210246/1‑89 (MQ=255) tCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTG‑TTAAT‑GGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCg < 2:169521/90‑1 (MQ=255) gACGGCATTATCGGCGGGGGGGGCGGGTATGAGCGGGTGGGTG‑TTTAT‑GGGGGTGGCGGGGGCGGGTTTTTTTTTCGGGGTTTTCGAAAg > 2:175753/1‑90 (MQ=255) gCATTATCGGCGGGGGCGGCGGGTATGAGCGGCTGGCTG‑TTTAT‑GGGGGTGGCGGGGGCTGTTTTTTTTTTCCGGGTTTTCGAAAGCTgg > 2:393840/1‑90 (MQ=255) ggcggGTGCGTCGGATATGAGCGGCTGGCTG‑TTAAT‑GGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCAt < 1:124603/90‑1 (MQ=255) gTGCGTCGGATATGAGCGGCTGGCTG‑TTAAT‑GGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGcc < 1:175753/90‑1 (MQ=255) gTGCGTCGGATATGAGCGGCTGGCTG‑TTAAT‑GGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGcc < 1:656028/90‑1 (MQ=255) gTGCGTCGGATATGAGCGGCTGGCTG‑TTAAT‑GGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGcc < 2:300283/90‑1 (MQ=255) gctggctg‑TTAAT‑GGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGa > 2:887322/1‑90 (MQ=255) | GGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTG‑TTAAT‑GGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGA > NZ_CP009273/1074871‑1075022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |