| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 55.0% | 13.7 / 25.2 | 21 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (1/8); new base G (11/0); total (13/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACC‑TTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152638‑1152816 | gCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGt < 2:670170/90‑1 (MQ=255) tGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGGAATTACTgg > 2:797361/1‑90 (MQ=255) aagaaAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACa < 1:347516/90‑1 (MQ=255) gCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACC‑t < 2:608287/90‑1 (MQ=255) gTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACC‑TTc < 1:475214/90‑1 (MQ=255) cTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACC‑TTCCGTAATGc < 2:331968/90‑1 (MQ=255) cTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATACTGGATCTCGGGTTTTTTATCTCCTTTTTCCGCATTTTGACC‑TTCCCTAATGc > 1:331968/1‑90 (MQ=255) aggTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACC‑TTCCGTAATGc < 1:373617/88‑1 (MQ=255) gTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTg < 1:891568/38‑1 (MQ=255) gTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTg > 2:891568/1‑38 (MQ=255) gTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATACTGGATCTCGGGTTTTTTATCTCCTTTTTCCGCATTTTGACC‑TTTCCTAAAGCgg > 1:330151/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATAATTGATCTCCGGTTTTTTAATTCCTTTTTTCGCCTTGTGGCC‑TTCCGTAATgggg > 1:956776/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATAATGGATCTCGGGTTTTTAATCTCCCTTTTCCGCATTTTTACC‑TTCCCTAATgggg > 1:377993/1‑90 (MQ=255) cAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCCCCTTTTCCGGCATTGTGACC‑TTCCGTAATGCGGAGCAAc < 1:1121493/90‑1 (MQ=255) gagGACAGAGAAACGGC‑GGGGAAAATACTGGGTTTCGGGTTTTATATCTCCTTTTTCGGCATTTTGACC‑TTCCCTAATGCGGGGGAACTg > 1:45414/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATTATTGG‑TCTCGGGTTTTTAATTTCCTTTTTCCGCGTTTTGACC‑TTCCGTAATGCGGGGGAACAgcg > 1:203949/1‑90 (MQ=255) ggACAGAGAAACGGC‑GGGGAAAATAATTGGTTTCGGGTTTTTTATTTTCTTTTTCCGCCTTGTGGCCTTTCCGT‑ATTCGGGGGAACTgcg > 1:884471/1‑90 (MQ=255) ggACAGAGAAACGGC‑GGGGAAAATAATTGATCTCGGGTTTTTAATCTCCTTTTTCCGCGTTTTGGAC‑TTCCGTAATTCGGGGGAAATgcg > 1:409473/1‑90 (MQ=255) ggACAGAGAAACGGC‑GGGGAAAATAATGGGTCTTGGGTTTTTCATCTCCTTTTTCGGCATTGTGGCC‑TTCCCTAATGCGGGGCAACTgct > 2:373617/1‑89 (MQ=255) ggACAGAGAAACGGC‑GGGGAAAATAATGGGTCTCCGGTTTTTTATCTCCCTTTTCTGCATTTTGACC‑TTCCGTAATGCGGGGCAACAgcg > 1:1211380/1‑90 (MQ=255) gagaAACGGC‑GGGGAAAATACTGGGTCTCGGGGTTTAAAACTCCTTTTTCCGCCTTTTGACC‑TTCCCTAATGCGGGGGAACCGCGCGGtg > 1:413122/1‑90 (MQ=255) gaAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACC‑TTCCGTAATGCGGAGCAACTGCGCGAtgct < 2:956776/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACC‑TTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:62143/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACC‑TTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:292416/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACC‑TTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:797361/90‑1 (MQ=255) | GCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACC‑TTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152638‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |