| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,254,452 | 0 | T | G | 60.0% | ‑0.5 / 10.3 | 20 | V69G (GTG→GGG) | ychH | stress‑induced protein YchH |
| Reads supporting (aligned to +/- strand): ref base T (4/4); new base G (12/0); total (16/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.89e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAA > NZ_CP009273/1254371‑1254525 | tCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGAcc < 2:804906/90‑1 (MQ=255)tCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGGGTGCGAcc > 2:1125284/1‑90 (MQ=255)tCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGGTGGCGGGCATGAACAGGTGTGCGAcc > 1:366211/1‑90 (MQ=255)tCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGGGCCCGTGTTGGCGGGCATGAACAGGGGTGCGAcc > 1:279484/1‑90 (MQ=255)tCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGGGCCCGTGTTGGCGGGCATGAACAGGGGTGCGAcc > 2:1150030/1‑90 (MQ=255) gTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGGGCCCGTGTTGGCGGGGGTGAAAAAGGGGGGGGCCGTTACTGGTggggt > 1:1004935/1‑88 (MQ=255) gCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCg < 1:455114/90‑1 (MQ=255) aaGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTTCTGGTGGGGTCGCCa > 2:14375/1‑90 (MQ=255) aaGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGGTCGCCa > 2:372879/1‑90 (MQ=255) aaGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGGGTGCGACCGTTACTGGGGGGGTCGccc > 2:1084339/1‑89 (MQ=255) aaGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGGGGGCGACCGTTTCTGGGGGGGTCGccc > 1:993621/1‑89 (MQ=255) ttttCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGGGTGCGACCGTTACTGGGGGGGTCGCCCCTATg > 2:538325/1‑90 (MQ=255) ttttCGTCGGTGCCATTCTCTGGCTGGCGGGGGCCCGTGTTGGCGGGCATGAACAGGGGTGCGACCGTTACTGGTGGGTTCGCCACTATg > 2:740082/1‑90 (MQ=255) ttttCGTCGGTGCCATTCTCTGGCTGGCGGGGGCCCGGGTTGGCGGGGATGAAAAGGGGGGCGGCCCTTACTGGGGGGGTCGCCCCCATg > 1:1160885/1‑90 (MQ=255) ttCGTCGGTGCCATTCTCTGGCTGGCGGGGGCCCGTGTTTGCGGGGCTGAAAAGGGGTGCGGCCGGTTCTGGGGGGGTCGCCCCTATGAc > 1:622923/1‑90 (MQ=255) gtcgGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGGGGGCATGAAAAGGGGTGCGACCGGTACTGGGGGGGTCGCCACTATGACaaa > 2:660025/1‑90 (MQ=255) cgGTGCCATTCTCTGGCTGGCGGGTGGCCGTGTTGGCGGGGATGAACAGGGGTGCGACCGTTACTGGGGGGGTTGCCACTATTACAAACg > 1:1045032/1‑90 (MQ=255) ggcGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGAcc < 1:331442/41‑1 (MQ=255) ggcGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGAcc > 2:331442/1‑41 (MQ=255) ggCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTaa < 2:1045032/90‑1 (MQ=255) | TCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAA > NZ_CP009273/1254371‑1254525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |