| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,548,257 | 0 | T | G | 55.0% | 14.0 / 16.8 | 20 | Y557S (TAC→TCC) | maeA | malate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base G (11/0); total (14/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.17e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTG > NZ_CP009273/1548174‑1548346 | gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTTgg > 2:768243/1‑90 (MQ=255)gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGGCGCGGGATTCgg > 2:1138892/1‑90 (MQ=255)gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGTCGCGGGATTCgg > 2:793983/1‑90 (MQ=255) gAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGc > 2:670388/1‑90 (MQ=255) agagGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTa < 1:162619/90‑1 (MQ=255) agGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATc < 2:289774/90‑1 (MQ=255) gCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGGCGCGGGATTTGGCTTGCCCGAAAATATCGTCAAAGGc > 1:34065/1‑90 (MQ=255) ggTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGt > 2:32380/1‑90 (MQ=255) gCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCa < 2:308240/90‑1 (MQ=255) gggCGCAGGCTTAGATGGAGGTACGGCGGGAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAAAGGCCTGTTGCAGGGGTTCGGccg > 1:367606/1‑88 (MQ=255) gcgcAGGCTTAGATGGAGGTACGGCGGGAGTCGCGGGATTCGGGTTGCCCGAAAATATTGTCAAAGGGCTGTTGGCGGGGTTCGGcgggg > 1:892970/1‑86 (MQ=255) ggAGGTCGGGCGGTAGTCGCGGTATCCGGCTTGCCAGAAATTATCGTAAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCAc < 2:1009106/90‑1 (MQ=255) gAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAATATCGTCAATGGCCCGTTGCAGGGCTTCGGGAGAGGGTTTTTCCGCCCCg > 1:241739/1‑90 (MQ=255) gAGGTACGGCGGTAGTCGCGGGATTTGGGTTTCCAGAAAATATTGTCAAAGGGCTGGTGCAGGGGTTTGGCAGAGGGTTTTACCGccccc > 1:1009106/1‑87 (MQ=255) gAGGTACGGCGGTAGTCGCGGGATTTGGCTTGCCAGAAAATATCGTCAATGGGCCGTTTGAGGGGTTCGGCAGAGGGTTTTACCGccccc > 1:127646/1‑87 (MQ=255) gAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAAAGGCCTGTTGCCGGGCTTCGGCAGAGGGTTTTACCGCCACg > 2:1239786/1‑90 (MQ=255) aCGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGCCCCGCCTtg > 2:1120996/1‑90 (MQ=255) gggTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTtgctgc < 1:282170/89‑1 (MQ=255) cgGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCAc > 1:981733/1‑71 (MQ=255) cgGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCAc < 2:981733/71‑1 (MQ=255) tATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTg > 1:477233/1‑90 (MQ=255) | GATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTG > NZ_CP009273/1548174‑1548346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |