| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,819,313 | 0 | G | A | 64.3% | ‑4.8 / 11.2 | 14 | intergenic (‑119/+84) | ves/spy | environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy |
| Reads supporting (aligned to +/- strand): ref base G (5/0); new base A (0/9); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT > NZ_CP009273/1819233‑1819401 | ggTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTCGAGAAGAgaaaagaaaa > 1:1103370/1‑90 (MQ=255) tGGTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTg < 2:1146208/90‑1 (MQ=255) aTCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCAc < 2:168725/90‑1 (MQ=255) aTCGGAAACTCGGGTGATTTACGTTAGTGGTGATCAGTTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCAc < 2:606585/90‑1 (MQ=255) aaCTGGGTTAATTTATGTTAGTGGCGACCCGGTTTTTTTTACAATTGTTTGAAAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCAtt < 1:411920/90‑1 (MQ=255) aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa > 1:1082711/1‑90 (MQ=255) ctggggTGTTTTACGTTAGTGGTGATCGGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTAc < 2:725877/87‑1 (MQ=255) ggTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCa > 1:1171146/1‑90 (MQ=255) gTGATTTACTTTAGTGGTGATCGGTTTTTTTTAAAAACTTTTAGAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCaa < 1:126748/90‑1 (MQ=255) gTGATTTACGTTAGTGGTGATCAGGTTTTTTTTAAAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:885212/90‑1 (MQ=255) aTCAGGTTTTTTTTACAATTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:1171146/90‑1 (MQ=255) tGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCt > 1:607875/1‑90 (MQ=255) tGTTTGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCt < 2:1082711/90‑1 (MQ=255) gTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg > 1:341441/1‑90 (MQ=255) ttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGaa > 1:381419/1‑90 (MQ=255) agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGattatt > 1:1107241/1‑90 (MQ=255) | GGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATT > NZ_CP009273/1819233‑1819401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |