| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,155,570 | 0 | T | G | 54.2% | 29.2 / 34.6 | 24 | V209G (GTA→GGA) | mdtD | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/11); new base G (13/0); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.01e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCTGTTAATGCCGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCTGGCGCTGGACGGCAGTAAAGGTACAGGTTTATCGCCGCTGACGATTGCAGGCCTGGTCGCAGTTGGC > NZ_CP009273/2155489‑2155646 | tGCTGTTAATGCCGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGA‑TTAAccc > 2:976817/1‑90 (MQ=255) gCTGTTAATGCCGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCt < 1:149338/90‑1 (MQ=255) tAATGCCGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGATTTAACCCCgggg > 1:899338/1‑88 (MQ=255) tAATGCCGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGATTTAACCCCgggc > 1:905314/1‑88 (MQ=255) aaTGCCGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCTGGCGCt < 1:587627/90‑1 (MQ=255) tGCCGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGA‑TTAAACCTGGGGCTgg > 2:382389/1‑90 (MQ=255) cGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCTGGCGCTGGACg < 2:536393/90‑1 (MQ=255) aaCTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGA‑TTTACCCCCGCGCTGGGCggg > 1:13412/1‑89 (MQ=255) aCTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCTGGCGCTGGACGGCa < 2:643453/90‑1 (MQ=255) acCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCTGGCGCTGGACGGCAGTaaa < 2:294790/90‑1 (MQ=255) acCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCTGGCGCTGGACGGCAGTaaa < 2:709544/90‑1 (MQ=255) acCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCTGGCGCTGGACGGCAGTaaa < 2:817790/90‑1 (MQ=255) aTGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGA‑TTTAACCCGGGGCCGGGCGGCCGGAAAggg > 1:849730/1‑89 (MQ=255) tGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGA‑TTAACCCTGGCGCTGGACGGCAGTAAAggga > 2:310966/1‑88 (MQ=255) cgcgGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGCGGGGT‑TTAACCCGGGGCCTGG‑CGGCCGGAAAGGGACAgggt > 1:611250/1‑88 (MQ=255) ggcgCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGA‑TTAACCCTGGGGCTGGGCGGCAGTAAAGGGACAGGgtttt > 2:801107/1‑88 (MQ=255) ggcgCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGA‑TTAAACCCGGGGCTGGGCGGCCGGAAAAGGACAGGGTTAt > 1:197029/1‑90 (MQ=255) ggcgCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGA‑TTAAACCCGGGGCTGGGCGGCAGGAAAAGGACAGGgtttt > 2:918925/1‑88 (MQ=255) gcgcTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGA‑TTAACCCTGGCGCTGGGCGGCAGTAAAAGGACAGGGTTATc > 2:955077/1‑90 (MQ=255) ttGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCTGGCGCTGGACGGCAGTAAAGGTACAGGTTTATcgccgc < 2:660935/90‑1 (MQ=255) ctcCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGGA‑TTAACCCTGGCGCTGGACGGCAGTAAAGGGACAGGGTTATCGCCGCTGACGAt > 2:1143246/1‑90 (MQ=255) ttGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCTGGCGCTGGACGGCAGTAAAGGTACAGGTTTATCGCCGCTGACGATTGCAGGCCTGGTc < 1:972941/90‑1 (MQ=255) ttGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCTGGCGCTGGACGGCAGTAAAGGTACAGGTTTATCGCCGCTGACGATTGCAGGCCTGGTc < 1:382389/90‑1 (MQ=255) gTTGGCATGGC‑GGTA‑TTAACCCTGGCGCTGGACGGCAGTAAAGGTACAGGTTTATCGCCGCTGACGATTGCAGGCCTGGTCGCAGTTGGc < 2:874128/90‑1 (MQ=255) | TGCTGTTAATGCCGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGC‑GGTA‑TTAACCCTGGCGCTGGACGGCAGTAAAGGTACAGGTTTATCGCCGCTGACGATTGCAGGCCTGGTCGCAGTTGGC > NZ_CP009273/2155489‑2155646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |