| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,999,337 | 0 | C | A | 66.7% | ‑2.3 / 14.5 | 18 | intergenic (‑192/‑287) | ygeV/ygeW | sigma‑54‑dependent Fis family transcriptional regulator/knotted carbamoyltransferase YgeW |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base A (12/0); total (15/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATT > NZ_CP009273/2999263‑2999426 | ttttCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTTTTAAAATGAAGAGAGATAGcc > 2:125974/1‑90 (MQ=255) tCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGGGGTTTTTAAAATGAAGAAAGATAAACTGc > 1:986590/1‑90 (MQ=255) gAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCttt < 2:1113367/90‑1 (MQ=255) gaTATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGAt < 2:1220587/90‑1 (MQ=255) tttCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAAATGAAGAGAGATAACCTGCCCCTTTTTCTTTTTTTTGATACTTagcag > 1:275637/1‑90 (MQ=255) ttCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTTTGATACTTagcagc > 2:402710/1‑90 (MQ=255) tataTCAATATGAGAATTACGGCGGTGAGGTTTTTAAAATTAAGAAAGATAACCTGCCCCCTTTTTTTTTTTTTGTTACTTTGCAGCaaa > 1:555415/1‑90 (MQ=255) atatCAATATGAGAATTACGGCGGTGGGGTTATTAAAATGAAAAGAGATAGCCCGCCCCCTTTTTTTTTTTTTTATACTTTGCAGCaaaa > 1:72768/1‑89 (MQ=255) atCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTa < 1:354546/66‑1 (MQ=255) atCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTa > 2:354546/1‑66 (MQ=255) tCAATATGAGAATTACGGCGGTGGGGTTTTTAAAATTAAAAGAGAAAAACTGCCCCCTTTTTTTTTTTTTTTTAATTAGAAGCaaaaaaa > 1:579097/1‑90 (MQ=255) tCAATATGAGAATTACGGCGGTGAGGTTATTAAAATTAAGAGAGAAAACCTGCCCCCTTTTTTTTTTTTTTATACTTTACAACaaaaaaa > 1:576735/1‑90 (MQ=255) aaTATGAGAATTACGGCGGTGAGTTTATTAAAATGAAAAGAGATAGCCTGCCCCCTTTTTTTTTTTTTTATACTTAGCAGCaaaaaaaaa > 1:303853/1‑88 (MQ=255) atatGAGAATTACGGCGGTGAGGTTATTAAAATGAAGAGAGATAGCCCGCCCCCTTTTCTTTTTTTTGATACTTTGCAGCAAataaaaaa > 2:555104/1‑90 (MQ=255) gagaATTACGGCGGTGAGGTTATTAAAATGAAGAGAGAAAGGCGGCCCCTTTTTTTTTTTTTTGATACTTAGCAGAAAAAAAAAAAcgcg > 1:189816/1‑90 (MQ=255) gaATTACGGCGGTGGGGTTTTTAAAAAGAAGAGAGAAAACCTGCCCCCTTATCTTTTTTTTTATACTTAGCAGCAAATAAAAAAAGCGaa > 1:516417/1‑89 (MQ=255) gaATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTTTTTTATTTTTGATACTTAGCAGCAAATAAAAAACGCGAt > 2:1211249/1‑90 (MQ=255) aCGGCGGTGGGGTTTTTAAAATGAAGAGAGATAGCCTGCCCCCTTTTTTTTTTTTTGTTAATTTGCAGCAAAAAAAAAAAACGATaaaaa > 1:1007126/1‑90 (MQ=255) cTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTAtt > 2:560824/1‑90 (MQ=255) | TTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATT > NZ_CP009273/2999263‑2999426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |