Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,152,7270TG54.5% 9.6 / 13.5 11G165G (GGT→GGG) ycfHmetal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGG‑ATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGT  >  NZ_CP009273/1152640‑1152811
                                                                                       |                                                                                     
cGATACGCTGGCAATTCTGCTCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTaa                                                                                     <  2:118600/90‑1 (MQ=255)
       cTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTg                                                                              <  2:467707/90‑1 (MQ=255)
                                            gTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGG‑ATTTTACATCTCCTTTTCCGGCATTGTGa                                        <  1:224025/90‑1 (MQ=255)
                                                           gTTTTACAGAGGACAGAGAAACGGCGGGGGAATTTATTGAAATCGGCGTTTTAAACTTCCTTTTCC‑TCATTTTTGACTTCCCTAATGCgg                         >  2:244666/1‑90 (MQ=255)
                                                              ttACAGAGGACAGAGAAACGGCGGGGAAAATAATGGGTCTCGG‑GTTTTACATCTCCTTTTTCGGCCTTGTGGGCCTCCCTAATGCggggg                      >  1:88167/1‑87 (MQ=255)
                                                                   gagGACAGAGAAACGGCGGGGAAAATACTGGGTCTCGG‑GTTTTAAATCTCCTTTTTCGGCATTTTGACCTTCCGTAATGCGGGGCAAccg                 >  1:447270/1‑88 (MQ=255)
                                                                   gagGACAGAGAAACGGCGGGGAAAATAATGGATCTCGG‑GTTTTACATCTCCCTTTTCGGCCTTGTGACCCTCCGTAATGCGGAGGAACTg                 >  1:141772/1‑90 (MQ=255)
                                                                      gACAGAGAAACGGCGGGTAAATTACTGGATCTCGG‑ATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTgcgc              <  2:278234/90‑1 (MQ=255)
                                                                        cAGAGAAACGGCGGGGAAAATACTGGATCTCCG‑GTTTTACAACTCCTTTTTCGGGATTTTGGCCTTCCCTAATGCGGGGGGACTGCGCGa            >  1:327342/1‑90 (MQ=255)
                                                                                  gcggGTAAATTACTGGATCTCGG‑ATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGt  <  1:244666/90‑1 (MQ=255)
                                                                                  gcggGGAAATTACTGGGTCTCCG‑GTTTTTCATCTCCTTTTCCCGCATTGTGACCTTCCCTTATGCGGGGCAACCGCGCGAGGCTGCGCGt  >  2:348361/1‑90 (MQ=255)
                                                                                       |                                                                                     
CGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGG‑ATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGT  >  NZ_CP009273/1152640‑1152811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑