Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,832,7180TG54.5% 9.3 / 10.7 11intergenic (‑95/‑165)ascG/ascFDNA‑binding transcriptional regulator AscG/PTS cellobiose/arbutin/salicin transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base G (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAA  >  NZ_CP009273/2832649‑2832789
                                                                     |                                                                       
aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGa                                                     <  1:336091/90‑1 (MQ=255)
     aGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAAAATTCAGGGGACCGGGTTCACAAATATAAAAAAAGAACAAt                                                >  2:348095/1‑90 (MQ=255)
       tgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGGGACCGGGTTCACAAATATAAAAAATGAACAATTc                                              >  1:355847/1‑90 (MQ=255)
                  gTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAATATTCAGGGGACCGGGTTTACAAATATAAAAAAAGAACAATTCACTCTCTTGCt                                   >  2:370212/1‑90 (MQ=255)
                  gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTTCt                                   >  1:470172/1‑90 (MQ=255)
                  gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt                                   >  1:193767/1‑90 (MQ=255)
                           ttGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTg                          <  2:65623/90‑1 (MQ=255)
                                cTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                     <  1:370212/90‑1 (MQ=255)
                                       aaTCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCAt              >  2:55433/1‑90 (MQ=255)
                                        aTCGGCGGGAAAAATATTTAGGTGACCGGGTTCACAAATATAAAAAATGAAAAATTCACTCTCTTGCTTTTTTAGTGACAACTATTCATg             >  2:461533/1‑90 (MQ=255)
                                                   aaaTATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaaa  <  2:101144/90‑1 (MQ=255)
                                                                     |                                                                       
AACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAA  >  NZ_CP009273/2832649‑2832789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑