| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,268,928 | 0 | G | C | 55.6% | 6.3 / 10.6 | 9 | A96A (GCG→GCC) | garD | galactarate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base C (0/5); total (2/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAGTGATTGGTTACGCCGTGCGTGCAATCCCACGCGGAAGCTGGATCGACGAATCAATGGTTGTACTACCGGAAGCGCCGCCGTTACACACGCTGCCACTGGCAACCAAAGTCCCGGAACCCTTACCGCCGCTGGAAGGATACACCTTTGAGGGCTATCGCAA > NZ_CP009273/3268851‑3269014 | gAAGTGATTGGTTACGCCGTGCGTGCAATCCCACGCGGAAGCTGGATCGACGAATCAATGGTTGTACTACCGGAAGCGCCGCCGTTacac < 2:371907/90‑1 (MQ=255) gTTACGCCGTGCGTGCAATCCCACGCGGAAGCTGGATCGACGAATCAATGGTTGTACTACCGGAAGCGCCGCCGTTACACACGCTGCCAc < 1:185756/90‑1 (MQ=255) aCGCCGTGCGTGCAATCCCACGCGGAAGCTGGATCGACGAATCAATGGTTGTACTACCGGAAGCGCCGCCGTTACACACGCTGCCACTgg > 2:191724/1‑90 (MQ=255) gtgcgtgcAATCCCACGCGGAAGCTGGATCGACGAATCAATGGTTGTACTACCGGAAGCGCCGCCGTTACACACGCTGCCACTGGCAAcc > 2:229707/1‑90 (MQ=255) cgacaaATAAATGTTTGTCCTCCGGGAGGCCCCCCCGTTACACACGCTGCCACTGGCAACCAAAGTCCCGGAACCCTTACCGCCGCTGGa < 1:307672/90‑1 (MQ=255) aaTGTTTGTACTCCCGGAAGCCCCGCCGTTACACACGCTGCCACTGGCAACCAAAGTCCCGGAACCCTTACCGCCGCTGGAAGGATacac < 1:146297/90‑1 (MQ=255) tGTACTCCGGGAAGCCCCGCCGTTACACACGCTGCCACTGGCAACCAAAGTCCCGGAACCCTTACCGCCGCTGGAAGGATACACCTTTGa < 1:191724/90‑1 (MQ=255) aGCCCCGCCGTTACACCCGCTGCCACTGGCAACCAAAGCCCCGGACCCCTTACCGCCGCTGGAAGGATACACCTTTGAGGGCTATCGCaa < 1:281117/90‑1 (MQ=255) aGCCCCGCCGTTACACACGCTGCCACTGGCAACAAAAGTCCCGGACCCCTTACCGCCGCTGGAAGGATACACCTTTGAGGGCTATCGCaa < 2:34299/90‑1 (MQ=255) | GAAGTGATTGGTTACGCCGTGCGTGCAATCCCACGCGGAAGCTGGATCGACGAATCAATGGTTGTACTACCGGAAGCGCCGCCGTTACACACGCTGCCACTGGCAACCAAAGTCCCGGAACCCTTACCGCCGCTGGAAGGATACACCTTTGAGGGCTATCGCAA > NZ_CP009273/3268851‑3269014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |