| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 536,014 | 0 | T | G | 61.5% | 5.5 / 11.7 | 13 | intergenic (+50/‑7) | allB/ybbY | allantoinase AllB/uracil/xanthine transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (8/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTT > NZ_CP009273/535926‑536098 | gcgcCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgtg < 2:31878/90‑1 (MQ=255) gcCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTTAGgggtg > 2:393576/1‑90 (MQ=255) gcCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgggtg > 2:8395/1‑90 (MQ=255) aTCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTg < 1:754790/90‑1 (MQ=255) tCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGGATTTTCCGGTTAAGGGGTGTTTTTGTTTAAtttttc > 2:448126/1‑88 (MQ=255) tCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCCGTTTAGGGGTGGTTTTGTTCAAtttttc > 1:451547/1‑88 (MQ=255) tCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGGGTTTATGTTCAAtttttc > 2:267492/1‑88 (MQ=255) aaaCATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGGGTTTATGTTCAATTTTTCAGTCa > 2:754790/1‑90 (MQ=255) cagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGaaa < 2:512293/90‑1 (MQ=255) gCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGGGTTTTTTTTTAATTTTTCAGTCAGCCGCGAAAGCCTGttttt > 2:463289/1‑87 (MQ=255) cggGCATTCTCCGGTTAAGGGGGGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGGTTTCAGTGGGTtttttttttt > 2:161020/1‑90 (MQ=255) ggggATTCTCCGGGTAAGGGGTGGTTATGTTTAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGGTTTCAGTGGGtttttttttttt > 1:108198/1‑90 (MQ=255) gggCATTCTCCGGTTAAGGGGTGTTTTTGTTTAATTTTGCAGGCAGCCGCGAAAGGCTGTTATTAGGGTTTTAGTGGTtttttttttttt > 1:444917/1‑90 (MQ=255) cATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTAtttttt < 2:720853/90‑1 (MQ=255) tcCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAac < 1:618807/90‑1 (MQ=255) tAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGtt < 2:563264/90‑1 (MQ=255) | GCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTT > NZ_CP009273/535926‑536098 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |