Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,380,3620CT56.2% ‑1.8 / 17.9 16intergenic (+52/‑51)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
Reads supporting (aligned to +/- strand):  ref base C (1/6);  new base T (9/0);  total (10/6)
Fisher's exact test for biased strand distribution p-value = 8.74e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGA  >  NZ_CP009273/2380278‑2380405
                                                                                    |                                           
ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCAAAAAAAAGACAAGCACAATTTTTTTTTCCTCa                                        >  2:4940/1‑90 (MQ=255)
  tgtTATAACTTTGTTATCCAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc                                      <  1:21228/90‑1 (MQ=255)
  tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc                                      <  1:679578/90‑1 (MQ=255)
  tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc                                      <  2:659989/90‑1 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGAAAAGAACATTTTTTTTTTTCTCATCtttttttaa                             >  1:504785/1‑87 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCCTCtttttttaa                             >  2:370050/1‑87 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCCTCTGtttttaa                             >  2:457942/1‑87 (MQ=255)
           tttGTTATACAACAGTAAATAATAAAAATTTCTCG‑GGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGa                            >  2:635952/1‑90 (MQ=255)
             tGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTATTTTTCCTCCTCTTTTTTaataa                           >  2:244353/1‑87 (MQ=255)
                 ataCAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGa                       <  1:192471/90‑1 (MQ=255)
                   acaacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGaaa                     <  2:271980/90‑1 (MQ=255)
                           aaaTAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTTTTTTTAAATGGGAAAAAAGaaaa             >  2:727201/1‑89 (MQ=255)
                           aaaTAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAACACATTTTTTTTTTCCTTCTCTTTTTTTAAATGAGAAAAAAGaaaa             >  1:508488/1‑89 (MQ=255)
                                ataaAAATTTCTCGAGGCGGGTCCAGAAAAAACGAAATGGACATTTTATTTTTTCTCATATTTTTTTATATGCGAAACAAGAAATTTaaa        >  1:250134/1‑89 (MQ=255)
                                     aaTTTCTCGAGGCGGGGCCCGAAAAAACGACATGCACATTTTATTTTTCCCCATCTGTTTTTAAATGGGAAAAAAGAAAATCAAaggagg   >  2:614672/1‑90 (MQ=255)
                                      aTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga  <  1:114521/90‑1 (MQ=255)
                                                                                    |                                           
GGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGA  >  NZ_CP009273/2380278‑2380405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑