| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,621,984 | 0 | A | C | 66.7% | ‑2.0 / 17.1 | 21 | V105G (GTC→GGC) | pdeF | cyclic‑guanylate‑specific phosphodiesterase PdeF |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base C (0/14); total (6/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTT > NZ_CP009273/2621895‑2622072 | gCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGa > 2:639237/1‑90 (MQ=255)gCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGa > 2:488516/1‑90 (MQ=255) aaaCACAATCTGCCAGAATATCCGCGGGAAAATTAAACGGG‑TACCCCCATGTGAAACTTTTTTACGCCGGGGAGTAAAGCCCCTTTAAcc < 1:488516/90‑1 (MQ=255) tGCCAAAATATACGCGGGGAAATTAAACGGT‑TTCCCCCTTGAAAACTTTTGTTCCCCCGGGGAGTAAAGCCCCTTTAACCGCCCCAACag < 1:217877/90‑1 (MQ=255) cgCCAGAATAACCGC‑GGGAAATTTAACGGGTTATCACCTTGGGAACCGTTGTTACGCGGGGGAGAAAAGCCCTTTTAACCGCCCCAACag < 2:189881/89‑1 (MQ=255) agacAGAATATACGGTGGGAAATTAAACGGG‑TATCCCCATGTGAAACGTTTTTCCGCGGGGGAGTAAAGCCCCTGTAACCGCCCCAACag < 2:693929/87‑1 (MQ=255) ccAGAATATCCGCGGGGAAATTAAACGGG‑TCTCCCCATGTGAAACGTTGTTGCGCCGGGGGGAAAAGACCCTGTAACCGCCCCAACagag < 2:636518/90‑1 (MQ=255) cAGAATATACGCTGGAAAATTAAACGGG‑TATCCCCATGTGAAACGTTGTTCCGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAgaga < 2:661285/90‑1 (MQ=255) gggAAATTAAACGGG‑TATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAAtgataatgataa > 1:349830/1‑90 (MQ=255) agggg‑TACCACCATGAAAAAATTTGTTAGGGCGGGGAAAAAAGCCCCTATAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGc < 1:310631/88‑1 (MQ=255) aCGGG‑TATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGc > 1:714300/1‑90 (MQ=255) tgtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGc > 1:728647/1‑90 (MQ=255) ttgttACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaa > 1:19482/1‑90 (MQ=255) ttgttACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaa > 2:253784/1‑90 (MQ=255) ttttACGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaaa < 2:523055/88‑1 (MQ=255) cgcgGGGGAAAAAAGCCCCTGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAccc < 1:203162/90‑1 (MQ=255) acgggggaAGTAAAGCCTCTGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAccc < 2:19147/86‑1 (MQ=255) gggggAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGc < 2:328975/89‑1 (MQ=255) gggggAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGc < 2:504427/89‑1 (MQ=255) ggggaAGAAAAGCCCTTGAACCCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGc < 1:610261/89‑1 (MQ=255) gggAGTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGctc < 2:685766/90‑1 (MQ=255) gggAGAAAAGCCCTTGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGctc < 2:472468/90‑1 (MQ=255) gACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCatctgt > 1:19147/1‑86 (MQ=255) | GCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTT > NZ_CP009273/2621895‑2622072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |