| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,171,099 | 0 | A | C | 60.0% | 17.6 / 23.1 | 20 | G57G (GGT→GGG) | nudF | ADP‑ribose diphosphatase |
| Reads supporting (aligned to +/- strand): ref base A (6/2); new base C (0/12); total (6/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.22e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGA > NZ_CP009273/3171014‑3171182 | tGGTGTCGTACGCGGCAATTCGAATCTGCTCAATCAGCACAACTTCATCACGCAATGGGTCAAAGGGTAGCAAGAATGCGGCGTGACcgc < 2:155127/90‑1 (MQ=255)tGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACcgc < 1:333381/90‑1 (MQ=255) ggTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACcgcg > 2:92323/1‑90 (MQ=255) cGAATCTGCTCAATCAGCACAACTTCATCCCGCCCTGGGTCAAAGGGTAGAAAGACGGCGGCGTGCCCGCGCTAAAAATTTTCCcgccgc < 1:753484/90‑1 (MQ=255) aaTCTGCTCAATCAGCACAACTTCATCCCCCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCAc < 2:59514/90‑1 (MQ=255) aTCTGCTCAATCAGCACAACTCCACCACCCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTCCCCGCCGCAcc < 1:378076/90‑1 (MQ=255) tCAACCACCACAACTTCACCACCCCCGGGGTCAAGGGGTAGCAAGCCTGCGGCGTGCCCGCGCTAAAAAATTCCCCGCCGCACCTCATGa < 1:394075/90‑1 (MQ=255) cAACCAGCACACCTTCACCCCCCACTGGGCAAAGGGGTAGCAAGACGGGGGCGTGCCCGCCCTAAAAAATTTCCCGCCGCACCTCATGAc < 1:92323/90‑1 (MQ=255) acggggTCAAGGGGTAGAAAGACTGGGGCGTGCCCGCGCAAAAAATTTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGa < 1:441877/87‑1 (MQ=255) aaggggTAGCAAGCCTGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaaa < 1:333167/87‑1 (MQ=255) aaggggTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaaa < 2:172265/87‑1 (MQ=255) aaaGGGTAGCAAGCCTGCGGCGTGCCCGCCCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaaa < 1:491653/90‑1 (MQ=255) aaaGGGTAGCAAGACTGGGGCGTCCCCGCTCCCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaaa < 2:352076/90‑1 (MQ=255) gTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtat > 2:629877/1‑90 (MQ=255) gTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtat > 2:672875/1‑90 (MQ=255) tAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATcctgt > 2:441877/1‑86 (MQ=255) tGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaaa < 2:586988/75‑1 (MQ=255) tGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaaa > 1:586988/1‑75 (MQ=255) gcggcgTGCCCGCGCTAAAAATTTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAAt < 2:399480/90‑1 (MQ=255) ggcgTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGa > 2:462361/1‑90 (MQ=255) | TGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGA > NZ_CP009273/3171014‑3171182 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |