| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 517,106 | 0 | T | G | 56.2% | 9.3 / 16.4 | 16 | V412G (GTA→GGA) | ybbP | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (9/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTGCCGATTGTCAGTGTGGTGGTTGTGCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGATGCTGCTGAATGTACTTCGCCGCATGACGCTGAAATCGCTGCCTCTGCGCCTGG > NZ_CP009273/517017‑517195 | tCTGCCGATTGTCAGTGTGGTGGTTGTGCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGgt < 2:148725/90‑1 (MQ=255) aGTGTGGTGGTTGTGCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGGGCGGGAGTGTTGGGTTTg > 1:402025/1‑90 (MQ=255) ggtggtTGTGCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGGAGTACTGGGTTTGCtgtg > 2:486/1‑90 (MQ=255) tgtgCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGGGGGGGGGTACTGGGTTTTCTGTGGGgggt > 1:661571/1‑90 (MQ=255) tgtgCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGGGCGGGGGGACTGGCTTTGCTGTGCggggg > 1:637107/1‑87 (MQ=255) tgtgCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGGGCGGGAGTACTGGCTTTTCTGTGCggggg > 2:706324/1‑87 (MQ=255) tgctgctgcTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTgc < 1:328432/90‑1 (MQ=255) ctgctCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTgctgggc < 1:748490/90‑1 (MQ=255) gctCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGGAGTACTGGCTTTTCTGTGCGGGGGgctggggtg > 2:432061/1‑90 (MQ=255) ggATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGAtgctg < 2:744930/90‑1 (MQ=255) ttAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGATGctgctg < 2:99641/90‑1 (MQ=255) tGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGGGCGGGAGTACTGGGTTTGCTGTGGGGGGTGGTGGGGTGGGTGCTGCTGGATGTAcc > 1:744930/1‑89 (MQ=255) gcagcaTGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGATGCTGCTGAATGTACTTc < 1:289072/90‑1 (MQ=255) gggCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTTCTGTGCGGGGGGGTGGGGTGGGTGGTGCTGAAAGTACTTCGCCGCCTGACGCTgg > 1:372661/1‑89 (MQ=255) gCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGATGCTGCTGAATGTACTTCGCCGCATGACGCTGAAATCGCt < 1:706324/90‑1 (MQ=255) gcgcGGGAGTACTGGCTTTTCTGTGCGGGGTGCTGGGGGGGGTGGTGCTGGATGTACTTCCCCCCCTGACGCTGAAATCGCTGCCTCTgc > 1:633392/1‑90 (MQ=255) gcgcGGGAGGACTGGCTTTTCTGTGCGGGGGGCTGGGGTGGGTGGTGGTGGATGTACTTCGCCGCCTGAAGCTGAAATCGCTGCCTCTgc > 1:212102/1‑90 (MQ=255) tagtaCTGGCTTTGCTGTGCGGTGTGCTGGGCTGGATGCTGCTGAATGTACTTCGCCGCATGACGCTGAAATCGCTGCCTCTGCGCCTgg < 2:633392/90‑1 (MQ=255) | TCTGCCGATTGTCAGTGTGGTGGTTGTGCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGATGCTGCTGAATGTACTTCGCCGCATGACGCTGAAATCGCTGCCTCTGCGCCTGG > NZ_CP009273/517017‑517195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |