| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,291,076 | 0 | A | C | 53.8% | 3.4 / 13.3 | 13 | V141G (GTG→GGG) | yraQ | permease |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAG > NZ_CP009273/3290990‑3291149 | aGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCt > 2:294711/1‑90 (MQ=255) aaGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGc > 2:112036/1‑90 (MQ=255) accccAGCGTCGCCGGGTTTAACCCCGGATTGCCCATCCAGAATGCCAGCGCCCCGCCCATCGACCCCTGTTGGCGACGCATTCccgccg < 1:181880/87‑1 (MQ=255) cGGTTTTAACCCCGGATTCCCCATCCAGAATGCCAGCGCCCCGCCCATCGACCCCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGc < 2:124643/90‑1 (MQ=255) ttAACCCCGGATTGCCCATCCAGAATGCCAGCGCCCCGCCCATCGACCCCTGTGGGCGACGCATTCCCGCCGCGACCGGAGCCGCAcagc < 1:42220/90‑1 (MQ=255) tAACACCGGATTGCCCACCCAGAATGCCAGCGCCCCCCCCATCGCCCCCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCAcagca < 1:581288/90‑1 (MQ=255) acacCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAgg > 1:452112/1‑90 (MQ=255) ccGGATTGCCCATCCAGAATGCCGGCGCCCCGCCCATCGACCCCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTac < 1:294711/90‑1 (MQ=255) ccGGATTCCCCACCCAAAATGCCAGCCCCCCGCCCATCGACACCTGTGGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTac < 1:639499/90‑1 (MQ=255) ggATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTacac > 1:90704/1‑90 (MQ=255) gAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCaa > 1:155288/1‑90 (MQ=255) cgcCCCCCCCATCGCCCCCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAg < 1:3848/90‑1 (MQ=255) cgcCCCCCCCACCCACCCCTGTGGGCGCCGCATCCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAg < 2:134146/90‑1 (MQ=255) | AGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAG > NZ_CP009273/3290990‑3291149 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |