| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,572,320 | 0 | C | A | 52.9% | 10.7 / 24.8 | 17 | L182F (TTG→TTT) | hsdM | type I restriction‑modification system methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (6/2); new base A (0/9); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGT > NZ_CP009273/4572248‑4572394 | gAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTAcc < 1:174846/90‑1 (MQ=255) tCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTgcc > 1:491630/1‑90 (MQ=255) gCGTGTGCCCACCAGGGTCGCCCAGATGATTGGTTTGAGTCTTAAAAAAGGGGTGGTTTTCAATAAAAAGGCCCGCCGTACCTGccgccg < 1:347787/90‑1 (MQ=255) gCGTGTCGCCATCAGGGTCGTCCAGATCTTTGGTTTGCAACTTAACATAGGGGTGGGCTTCAACAAAAAAGCCCGCCGTACCTGccgccg < 1:509284/90‑1 (MQ=255) gCGTGTCGCCATCAGGGTCGCCCAGATCTTTGGTTTGCGATTTAACATAGGGGTGGGCTTCAATAAAAAAGCCCGCCGTACCTGccgccg < 1:370573/90‑1 (MQ=255) gCGTGCCGCCATCAAGGTCGCCCAGATCTTTGGTTTGCGATTAAACATAGGGGTCGGTTTCAAAAAAAAACCCCGCCGTACCTGccgccg < 2:417564/90‑1 (MQ=255) tCGCCATCAAGGTCGTCCAGATCTTTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTcc < 1:55669/90‑1 (MQ=255) gtcCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCAcg > 2:655156/1‑90 (MQ=255) aTCTTTGTTTTGCGACTAAACATGGGGGCCGGTTTAAAAAAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTg < 2:42424/90‑1 (MQ=255) ttGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGt > 2:660609/1‑90 (MQ=255) ttGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGt > 1:313492/1‑90 (MQ=255) tgttttGCGACTAAACATGGCGGTGGGTTTCAATAAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGtt < 2:128103/87‑1 (MQ=255) ttGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTcag > 1:42424/1‑90 (MQ=255) tGCGCCTAAACATGGCGGTCGGCTTCAAAAAAAAACCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTcagc < 2:617568/90‑1 (MQ=255) tGCGACTTAACATAGGGGTCGGTTTAAAAAAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTcagg < 1:533473/90‑2 (MQ=255) aTAGCGGTCGGCTCAAAAAAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGaataat < 1:459500/90‑1 (MQ=255) gCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGt > 1:214226/1‑90 (MQ=255) | GAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGT > NZ_CP009273/4572248‑4572394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |