Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,141,2140TG53.8% 5.3 / 13.6 13G20G (GGT→GGG) yegDmolecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base G (7/0);  total (10/3)
Fisher's exact test for biased strand distribution p-value = 6.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGCT  >  NZ_CP009273/2141155‑2141303
                                                           |                                                                                         
gtgtTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTGCTGGGTGTGGGTTAACCCCCATTTCTGAAAAAGAAAAAcaag                                                             >  1:265769/1‑87 (MQ=255)
            tttGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGctgctg                                                 <  2:265769/90‑1 (MQ=255)
               gATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGGAAACCGCATTTGCTAAAAAAGGAAAAAGACAGCACGCTGCTGCCt                                              >  2:49554/1‑90 (MQ=255)
                    cGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGGGCGGGAAAACGCATTTGCTAAAAAAGGAAAAAGACAGCACGCCGCTGCCCTCCAt                                         >  1:143150/1‑90 (MQ=255)
                      gTACAGCAAACTGTTCAGTGGCGGTCATGCGGGACGGGAAAACCCATTTTCTAAAAAAAGAAAAAGAAAACACGCTGCTGCCTTCAATGc                                       >  1:432557/1‑90 (MQ=255)
                         cAGCAAACTGTTCAGTGGCGGTCATGGGTGGCGGGAAAACGCATTTTCGAAAAAAGGAAAAAAACAACACGCTACTGCCTTCAAAACttt                                    >  2:352858/1‑90 (MQ=255)
                                cTGTTCAGTGGCGGTCATGCGTGACGGGAAACCGCATTTTCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGcc                             >  2:51748/1‑90 (MQ=255)
                                cTGTTCAGTGGCGGTCATGCGGGACGGGAAAACCCATTTGCTAAAAAAGGAAAAAAACAGCACCCGGCGGCCCTCCATGCTTTTTGCGcc                             >  1:421788/1‑90 (MQ=255)
                                 tGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCa                            <  1:51748/90‑1 (MQ=255)
                                    tCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAAcg                         <  2:304368/90‑1 (MQ=255)
                                    tCAGTGGCGGTCATGCGTGACGGGAAAACGCATTTTCTAAAAAAGGAAAACGACAGCACGCCGCTGCCTTCAATGCTTTTCGCGCCCAcg                         >  1:13050/1‑90 (MQ=255)
                                                 tGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTaa            >  2:205770/1‑90 (MQ=255)
                                                          gTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGc   >  2:368878/1‑90 (MQ=255)
                                                           tAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGCt  >  1:395724/1‑90 (MQ=255)
                                                           |                                                                                         
GTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGCT  >  NZ_CP009273/2141155‑2141303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑