| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 737,562 | 0 | A | C | 55.6% | ‑5.6 / 17.9 | 18 | S151A (TCT→GCT) | dtpD | dipeptide permease DtpD |
| Reads supporting (aligned to +/- strand): ref base A (7/1); new base C (0/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCCAACCGCCGCCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAC > NZ_CP009273/737481‑737645 | tGCCAACCGCCGCCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGt > 2:306919/1‑90 (MQ=255) cgccgcCAGGCCAAAGCCCACCGCCCAGCTGTCCTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGCCCCCACGTTACccgc < 2:325568/90‑1 (MQ=255) ccAGGCCAAAGCCCATCGCCCGGCTGTCCTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGCCCCCACGTTACCCGCCGCAt < 2:590402/90‑1 (MQ=255) ggCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACa > 1:47473/1‑90 (MQ=255) caccGCCCGGCTGTCCTCTCCCGGGGCGAACCCACAGGCGATGGGGGCAAAAAAAGCCCCCCCGTTCCCCGCCGCATACATCAGCGAGaa < 2:661841/87‑1 (MQ=255) ccAGCTGTCCTCTTCCGGGGCGTAACCACAGGCGATAGGTGCGATAATAGCCCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGcc < 1:306919/90‑1 (MQ=255) cTGTCCTTTCCCGGGGCCAAACCCCAGGCGAAAGGGGCGATAAAAGCCCCCCCGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCa < 2:312151/90‑1 (MQ=255) tctTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGCCCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGAcgc < 1:36364/90‑1 (MQ=255) ctTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATc < 1:87980/65‑1 (MQ=255) ctTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATc > 2:87980/1‑65 (MQ=255) ctTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGAcgcg > 1:629288/1‑90 (MQ=255) tGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCg > 2:29138/1‑90 (MQ=255) ggCGTAACCACAGGCGATAGGTGCGATAATAGCCCCCCCGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt < 1:149797/90‑1 (MQ=255) ggCGAACCCACAGGCGATGGGGGCAAAAAAACCCCCCCCTTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGt < 2:629288/90‑1 (MQ=19) acaGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATa > 1:748609/1‑90 (MQ=255) gCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGc > 1:676159/1‑90 (MQ=255) aaataGCCCCCCCTTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAc < 2:676159/89‑1 (MQ=38) aaataGCCCCCATGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAc < 2:416247/89‑1 (MQ=38) | TGCCAACCGCCGCCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAC > NZ_CP009273/737481‑737645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |