| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 832,048 | 0 | T | G | 56.2% | ‑5.6 / 14.8 | 16 | V81G (GTC→GGC) | hcxB | hydroxycarboxylate dehydrogenase HcXB |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (9/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAC > NZ_CP009273/831973‑832130 | aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACCCCCCATgggg > 2:684120/1‑88 (MQ=255) cTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCTCGATGGCGATCgcgc > 1:21747/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCCCGATGGGGGTCGGGCCTTTTGTCAg > 1:414697/1‑90 (MQ=255) cACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCTCGATGGGGATCGCGCATTTTGGCAGGGcgcg > 1:179666/1‑90 (MQ=255) ccTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGc < 1:562195/90‑1 (MQ=255) tGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACCCCCGATGGCGAACGCGCCTTTGGTGAGGGCGCGGcac > 2:203529/1‑90 (MQ=255) tAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGc < 1:460705/90‑1 (MQ=255) aaCCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGCGATCGCGCATTTGGTCAGGGCGCGGCACATGAAGCg > 1:226157/1‑90 (MQ=255) ccATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGAt < 1:684120/90‑1 (MQ=255) ccAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCCCGATGGCGATCGCGCCTTTTGTCAGGGCGCGGCACATGAAACGATGGGGCTgg > 2:181045/1‑90 (MQ=255) gTTAAAGAGGCGGGGGGGGCGGGCACCCCCCCTGGGGGTCGCGCCTTTGGTGAGGGGGCGGGCCATGAAACGAGGGGGCTGGGGGGTgag > 2:787706/1‑90 (MQ=255) gTTAAAGAGGCGGGGGGGGAGGTCACCCCCGTTGGCGTTTGCGCGTTTGGTCCGGGCCCGGCGCACAAATCGGTGGCCCCGGGGATTgag > 1:648657/1‑90 (MQ=255) tAAAGAGGCGGGGGGGGCGGGGACCCTCGGTGGGGATCGCGCTTTTTGTCCGGGGCCGGGACCTGGACCGAGGGCGGTGGGGGTTgggga > 1:727949/1‑86 (MQ=255) gggCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGc < 2:179666/90‑1 (MQ=255) gcggcgGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAc < 2:414697/90‑1 (MQ=255) gcggcgGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAc < 2:648657/90‑1 (MQ=255) | ATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAC > NZ_CP009273/831973‑832130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |