| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,329,948 | 0 | T | G | 54.5% | ‑4.1 / 24.4 | 22 | intergenic (+24/‑140) | BW25113_RS25560/acnA | hypothetical protein/aconitate hydratase AcnA |
| Reads supporting (aligned to +/- strand): ref base T (2/8); new base G (12/0); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTA > NZ_CP009273/1329865‑1330027 | ggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCAt > 1:12833/1‑90 (MQ=255) ttttCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctt < 1:238534/90‑1 (MQ=255) gCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTa < 2:515273/90‑1 (MQ=255) gAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCACAAGGGTTCTCCTCttttttta > 1:665891/1‑86 (MQ=255) gAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCACAAGGGTTCTCCTCTTTTATCa > 1:452453/1‑90 (MQ=255) aTTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctt < 2:451450/48‑1 (MQ=255) aTTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCAtttgctt > 1:451450/1‑48 (MQ=255) aTTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCACAAGGGTTTTCCTCTTTTTTCAATTTTGGGTGTTTTc > 2:126445/1‑90 (MQ=255) aTTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTGCCCCAAGGTTTTTCCTCTTTTTTCAATTTGGGTTGTTATc > 2:572879/1‑90 (MQ=255) tCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTTCCACAAGGGTTCTCCTCTTTTTTCAATTTGGGTTGTTTTCAAATc > 2:242891/1‑90 (MQ=255) cTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGtt < 1:126445/90‑1 (MQ=255) cTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGtt < 1:572879/90‑1 (MQ=255) cTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCCCAAGGTTTCTCCTCTTTTTTTCATTTGGGGTGTTATCAAATCCtt > 2:362357/1‑90 (MQ=255) cTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCACAAGGTTTTTCCTCTTTTTTTAATTTTGGGTGTTATTAAATCGtt > 2:546286/1‑90 (MQ=255) cTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTTCCACAAGGGTTTTCCTCTTTTTTTAATTTGGGTTGTTATCAAATCGtt > 2:358384/1‑90 (MQ=255) tGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTGCCACAAGGTTTTTCCTCTTTTTTCAATTTGGGGTGGTTTCAAATCGTTACGCGATg > 2:478193/1‑90 (MQ=255) cccGCTGCGGCGGGGGTGTCATTTGCTTTTCCACAAGGGTTCTCCTCTTTTTTCAATTTGGGGTGTTTTCAAATCGTTACGCGGTGtttt > 2:245339/1‑89 (MQ=255) cGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgtg > 1:576890/1‑90 (MQ=255) cGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgtg > 1:137148/1‑90 (MQ=255) cTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGtt < 1:362357/90‑1 (MQ=255) cTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGtt < 1:358384/90‑1 (MQ=255) tGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTa < 1:245339/90‑1 (MQ=255) | GGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTA > NZ_CP009273/1329865‑1330027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |