| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,118 | 0 | C | A | 53.8% | ‑2.9 / 11.5 | 13 | A422A (GCC→GCA) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (5/1); new base A (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTA > NZ_CP009273/3214029‑3214191 | aTTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGcc > 2:576404/1‑90 (MQ=255) tGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCaa < 1:735214/90‑1 (MQ=255) aTAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCa > 2:702693/1‑90 (MQ=255) tttGCCAGGGAGATTTCCCGCCGGCGCGTACTGGGGGCCGG‑AACGCTCAATAACCCAAAAACGACCCGCATTAAACCGCcactgacactg < 1:110581/90‑1 (MQ=255) tttGCCAGGGAGATTTCCCCCCGGCGCGT‑CCGGGGGCCGGAAACCCAAAAAACCGCAAAAACGACCCGCTTTAACCCGCcactgacactg < 2:773303/90‑1 (MQ=255) tcccAGCGAGTTTTCCCCCCGGCGCGTCCGGGTGGCCGA‑ACCCCTAAAAAACCCAAAAACGACCCGCATTAAACCGCCactgacactgac < 2:274462/88‑1 (MQ=255) gCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTg > 2:221033/1‑90 (MQ=255) gCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTg > 2:382371/1‑90 (MQ=255) aagaTGTCCCGCCGGCGCGTACGGGGGGCCGG‑ACCGCTAAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGaa < 1:696053/89‑1 (MQ=255) ttGTCCCGCCGGCGCGTCTGGGGGGCGGG‑AACCCTAAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAg < 1:713349/89‑1 (MQ=255) ccgccgGCGCGTCGGGGGGGCCGA‑AACGCAAAAAAACGCAAAACCGCCCCGCTTTGACCCGCCACTGACACTGACCATTGAACAGTGTGa < 2:122171/90‑1 (MQ=255) ggcgcGTACTGGTGGGGGG‑AACGCAAAAAACCGCAAAACCGACCCGTATTGACCCGCCACTGACACTGACCATTGAACAGTGTGAACTgg < 1:773057/89‑1 (MQ=255) gCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcggcg > 1:484748/1‑90 (MQ=255) g‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTa > 1:361227/1‑90 (MQ=255) | ATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTA > NZ_CP009273/3214029‑3214191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |