| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 172,755 | 0 | A | G | 55.6% | ‑1.0 / 29.0 | 27 | T388A (ACG→GCG) | clcA | H(+)/Cl(‑) exchange transporter ClcA |
| Reads supporting (aligned to +/- strand): ref base A (2/10); new base G (15/0); total (17/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.82e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGAT > NZ_CP009273/172676‑172837 | ggCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTAt < 1:633269/90‑1 (MQ=255) tGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTTCTATTTCCg > 1:449694/1‑90 (MQ=255) aCTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATg < 1:139252/90‑1 (MQ=255) gtgCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAAT‑TCACCTTGAGGCGGGGGCGGTTTGTTTTTCCGGGATggggg > 1:463859/1‑90 (MQ=255) cTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGGGGTTTCTATTTTCGGGATgggggga > 2:405927/1‑88 (MQ=255) cTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTATTGCCGGGATgggggga > 1:48230/1‑88 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTGGTAt > 1:170887/1‑72 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTAt < 2:170887/72‑1 (MQ=255) ccGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTTCTTTTGCCGGGATGGGGGGATTACTgg > 1:312628/1‑90 (MQ=255) ccGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTGCTATTGCCGGAATGGGGGCATTACTgg > 1:50705/1‑90 (MQ=255) ccGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTGGTTTTGCGGGAGTTGGGGGATTACtcg > 2:512129/1‑88 (MQ=255) cGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTTCTATTGCCGGAATGGGGGGATTACTTGCGGCATc > 1:756244/1‑90 (MQ=255) tGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTTCTATTGCCGGGATGGGGGGATTACTGGCGGCGTCTAttt > 2:780335/1‑89 (MQ=255) ttGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTGCTATTGCCGGGATGGGGGGATTACTGGCGGGAACTATTtgcg > 2:274353/1‑90 (MQ=255) gCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTcgcgcg < 1:730827/90‑1 (MQ=255) gCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTcgcgcg < 1:136608/90‑1 (MQ=255) cGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTTCTTTTGCCGGGATGGGGGGATTACTGGCGGCATCTATTTTCGCGcc > 2:136608/1‑90 (MQ=255) gTTGAGCTGTTTCCGCAAT‑TCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGt < 2:463859/90‑1 (MQ=255) gAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTGCTTTTTCCGGGATGGGGGGATTACTGGCGGCCTCTATTCGCGCGCCGttt > 2:304194/1‑89 (MQ=255) gAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGAGGTTGCTATTGCCGGGATGGGGGGATTACTGGGGGCATCTATTTTCGCGCCGTTa > 2:730827/1‑90 (MQ=255) gCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTTGCGCGCCGGTAAc > 1:448464/1‑90 (MQ=255) cTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACg < 1:699788/90‑1 (MQ=255) cTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACg < 1:502267/90‑1 (MQ=255) ttCCGCAATATCACCTTGAGGCGGGGGCGTTTTCTTTTTCCGGGATGGGGGGATTACTGGCGGGCTCTTTTTGCGCGCCGTTTACggggg > 2:699788/1‑89 (MQ=255) ccGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATc < 1:780335/90‑1 (MQ=255) cAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCAtt < 2:50705/90‑1 (MQ=255) ggCGGGGACGGTTTCTATTGCCGGAATGGGGGGATTACTGGCGGCATCTATTTGCGCGCCGTTAACGGGGGGCATTTTGGTTCTGGAGAt > 2:218179/1‑90 (MQ=255) | GGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGAT > NZ_CP009273/172676‑172837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |