| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,393,722 | 0 | T | G | 60.0% | ‑2.3 / 11.1 | 15 | G202G (GGT→GGG) | hflC | protease modulator HflC |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGCGGCGCTGGGTATTGAAGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGA > NZ_CP009273/4393652‑4393805 | cAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGCGGCGCTGGGGATTGAAGTTGTCGATGTGc > 2:627542/1‑90 (MQ=255) gcgTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGCGGCGCTGGGGATTGAAGTTGTCGATGTGCGTATCa > 1:124571/1‑90 (MQ=255) gTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGCGGCGCTGGGTATTGAAGTTGTCGATGTGCGTATCAAg < 2:421981/90‑1 (MQ=255) gCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGCGGCGCTGGGGATTGAAGTTGTCGATGTGCGTATCAAGCAGATc > 2:526336/1‑90 (MQ=255) tGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGCGGGGCTGGGGATTGAAGGTGTCGGTGTGCGTATCAAACAAAGCaa > 2:637427/1‑90 (MQ=255) aaaGTTCCGGTCATCAACCCGAACAGTATGGCGGCGCTGGGGATTGAAGTTGTCGATGTGCGGATCAAGCAGATCAACCTGCCGACCGaa > 2:710508/1‑90 (MQ=255) ccGGTCATCAACCCGAACAGTATGGCGGCGCTGGGGATTGAAGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCt > 2:270648/1‑90 (MQ=255) catcaACCCGAACAGTATGGCGGCGCTGGGTATTGAAGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGc < 2:260350/90‑1 (MQ=255) ccGAACAGTATGGCGGCGCTGGGTATTGAAGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTAc < 1:758480/90‑1 (MQ=255) cGAACAGTATGGCGGCGCTGGGTATTGAAGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACa < 2:418708/90‑1 (MQ=255) cGAACAGTATGGCGGCGCTGGGTATTGAAGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACa < 2:51303/90‑1 (MQ=255) gcTGGGTATTGAAGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGa > 1:743870/1‑90 (MQ=255) gcTGGGGATTGAAGTTGTCGGTGGGCGTATCAAGCAGATCAACCTGCCGACCCAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGa > 1:406091/1‑90 (MQ=255) gcTGGGGATTGAAGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGa > 1:603317/1‑90 (MQ=255) gcTGGGGATTGAAGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGGGTCTGAAGCGATCTACAACCGTATGCGCGCCGa > 2:61292/1‑90 (MQ=255) | CAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGCGGCGCTGGGTATTGAAGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGA > NZ_CP009273/4393652‑4393805 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |