| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,500,285 | 0 | A | C | 66.7% | 4.5 / 12.2 | 15 | V18G (GTA→GGA) | ydcO | BenE family transporter YdcO |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (0/10); total (4/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCAC > NZ_CP009273/1500197‑1500368 | cATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATAc < 2:521659/90‑1 (MQ=255) ttGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGc > 1:728754/1‑90 (MQ=255) ccGGCGACAACCGCTGCTTGCCAGATTTTTGCCGCTGACCTGGGGTAGCCAATTAATCCCGCCAAAAACCCCGCCAGTAGCGTGGGTgga < 2:728583/90‑1 (MQ=255) ccGGCGAAATTCGCTGCTTCCAAATTTTTTCCCGCTAACGGGGGGTACCAAATAAATCCCCCCAAAACCCCCGCCAGTAGCGTGGGTgga < 1:168631/90‑1 (MQ=255) cGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTggag > 1:736677/1‑90 (MQ=255) ggCGAAAATCGCTGCTTGCAAAATTTTTGCCGCTGAAGGGGCGTACCAAATAAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTggagg < 1:267782/90‑1 (MQ=255) ttccccAGATTATTCCCGGTGACGGGGGGTAGCAAATAAAACCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGAc < 1:625540/86‑1 (MQ=255) ctcgCCAAATTATTGCCGCTAAAGGGGCGTAGCAAATAAATCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGAc < 2:903195/87‑1 (MQ=255) ttttttGCCGCTGAAGGGGGGTAGCAAATTAATCCCGCCAAAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTc < 2:696293/86‑1 (MQ=255) ttttttGCCGCTAACCTGGGGTACCAAATAAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTc < 2:522025/86‑1 (MQ=255) tGAACGGGCGTAGCCAATAAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGt < 2:358658/90‑1 (MQ=255) ccgggCGTAGCAAATTAACCCCGCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtat < 2:831029/87‑1 (MQ=255) aGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACa > 2:572972/1‑90 (MQ=255) aaaTAAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGc < 2:418930/89‑1 (MQ=255) aTTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCAc > 2:465087/1‑90 (MQ=255) | CATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCAC > NZ_CP009273/1500197‑1500368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |