| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,673,561 | 0 | A | C | 60.0% | 9.3 / 13.8 | 15 | Y293S (TAC→TCC) | ydgH | DUF1471 family protein YdgH |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/9); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCC > NZ_CP009273/1673476‑1673643 | aTCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACcac > 1:467900/1‑90 (MQ=255) ggCAACTACGGAAACATGACCGAAGTCCCCTATCAGGTTGCGAAACGTGCCGAGAAAAAGGGTGCTAATTATTCCCCCACCCCCCGCCAg < 1:613608/90‑1 (MQ=255) aCCGAATTCTCCTATCAGGTGGCAAACCGTGCCGCGAAAAAAGGTGATAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGtaat < 1:1008218/90‑1 (MQ=255) gAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAAc > 2:1008218/1‑90 (MQ=255) ggTTGCAAACCGTGCCGGAAAAAAGGGTGCTAAGTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 2:642975/90‑1 (MQ=255) ggTTGCAAAACGTCCCGCAAAAAAAGGTGCTAATTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 1:287837/90‑1 (MQ=255) ttGCGAACCGTGCCGCGAAAAAAGGTGCTAAGTATTACCCCATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAg < 2:712575/90‑1 (MQ=255) tGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGGACGTGGTAATAACCTGACCGTCAGCGCAGa > 1:909641/1‑90 (MQ=255) tGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGGAATAACCTGACCGTCAGCGCAGa > 1:712575/1‑90 (MQ=255) cGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc < 2:406584/90‑1 (MQ=255) cGAAACGTCCCGCGAAAAAGGGTTAAAAGTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc < 1:1038565/90‑1 (MQ=255) aaagggTGCAAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTAtt < 2:467900/86‑1 (MQ=255) gTGCTAATTATTCCCACACCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGtt < 2:970081/90‑1 (MQ=255) gatAAGTTTTCCCCCACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTAc < 1:1010974/88‑1 (MQ=255) aaGTATTACCACATCACCCGCCAGTGGCAGGAACGGGGTAATAAACTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTcc > 1:880799/1‑90 (MQ=255) | ATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCC > NZ_CP009273/1673476‑1673643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |