| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,153,677 | 0 | T | G | 56.2% | 10.7 / 15.1 | 17 | V604G (GTG→GGG) | mdtC | multidrug efflux RND transporter permease subunit MdtC |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (9/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTG > NZ_CP009273/2153602‑2153756 | tGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGgatga < 2:937326/90‑1 (MQ=255) cAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGatgatg > 2:506190/1‑90 (MQ=255) ttCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATc < 2:959737/90‑1 (MQ=255) ttCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGGTGGTGTTTTTc > 1:366298/1‑90 (MQ=255) ttCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGATGATGTTTATc > 2:51985/1‑90 (MQ=255) aTCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGGTGATGTTTTTTACCCTCAAGCCa > 1:1061400/1‑90 (MQ=255) ggATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAAc > 2:160871/1‑90 (MQ=255) ggATAATGTCACCGGCTTTACAGGCGGTTCGCGAGGGCACAGCGGGGTGATTTTTTTCACCCCCAAACCACGCGAGGAAAGCAGAGaaaa > 1:170880/1‑89 (MQ=255) ggATAATGTCACCGGCTTTACAGGCGGTTCCCGAGAGAACAGCGGGATGATGTTTATAACCCTCAAGCCACGCGGCGAACGCAGCGaaaa > 2:1064967/1‑89 (MQ=255) aTGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGgcgc < 2:552979/90‑1 (MQ=255) aTGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGgcgc < 2:700039/90‑1 (MQ=255) aTGTCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGGTGGTGTTTTTCACCCCCAAACCCCCCGCCGAACGCAGCGAAAAGgcgc > 1:585404/1‑90 (MQ=255) aTGTCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGATGGTGTTTTTCACCCCCCAACCCCGCGACGGAAGCCGGGAAAAGgggc > 2:725600/1‑90 (MQ=255) tCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAAAGGCgcagc > 2:561475/1‑90 (MQ=255) aCCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACAGCGCAGCaa > 2:915036/1‑90 (MQ=255) cGGTTCGCGAGGGGAGAGCGGGGGGATGTTTATGACCCCCAAGCCGCGCGACGAACGCAGCGAAACGGGGCAACACATTATCCACCGGCt > 1:806723/1‑90 (MQ=255) ggTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTg < 2:1061400/90‑1 (MQ=255) | TGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTG > NZ_CP009273/2153602‑2153756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |