| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,458,992 | 0 | C | G | 60.0% | 8.4 / 26.2 | 20 | G71G (GGC→GGG) | yfdC | formate/nitrite transporter family protein |
| Reads supporting (aligned to +/- strand): ref base C (0/8); new base G (12/0); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.94e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGG > NZ_CP009273/2458903‑2459081 | gAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGgc < 2:644394/90‑1 (MQ=255) ccAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACt < 2:1014894/90‑1 (MQ=255) cAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGGCTGTCGGTGGGGGCTTCGTTTCtt > 2:717377/1‑89 (MQ=255) tGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCaaa < 1:941179/90‑1 (MQ=255) tGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCaaa < 2:109150/90‑1 (MQ=255) gTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGGGTGTGGGTGGGGGCCTCGTTTCTTGCcaaaa > 1:636147/1‑89 (MQ=255) gTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGGGTGTCGGGGGGGGGGTCGTTTCTGTCGAAAg > 1:718206/1‑90 (MQ=255) aaaaaGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGa < 2:235857/90‑1 (MQ=255) aaCGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCg < 1:589785/90‑1 (MQ=255) gcgACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGGCTGTCGGTGGGGGCTTCGTTTCTGGCAAAAAGGAGATTTTTAATCGAAc > 1:142427/1‑90 (MQ=255) aaTGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAgg < 1:847451/90‑1 (MQ=255) tgtgGTCAGCCATTGCGGCGGGGCTGTCGGTGGGGGCCTCGTTTCTGGGGAAAGGGGGATTTTAAGTTGGACTGGGAGGGGTGGCGGGCa > 1:765466/1‑90 (MQ=255) tgGTCAGCCATTGCGGCGGGTCTGTCGATGGGGGCTTCGTTACTGGCAAAAGGGATATTTTAAGTCGAACTGGAAGGGGGGGCGGGCAGc > 2:991880/1‑90 (MQ=255) ggTCAGCCATTGCGGCGGGGCTGTCGATGGGGGCTTCGTTTCTGGGAAAAAGGGGATTTTAAGTCGAACTGGGAGGGGGGGCGGGGAGct > 1:242609/1‑90 (MQ=255) ggTCAGCCATTGCGGCGGGGCTGTCGATGGGGGCTTCGTTTCTGGCAAAAAGGGTATTTTAAGTCGGAATGGGAGGGGGGGCGGGCCGct > 1:957039/1‑90 (MQ=255) cAGCCATTGCGGCGGGGCTGTCGGTGGGGGCTTCGTTTCTGGCAAAAAGGGTATTTTAAGTCGGAATGGGAGGGGGGGCGGGCAGcttct > 1:85641/1‑90 (MQ=255) cAGCCATTGCGGCGGGGCTGTCGATGGGGGCTTCGTTACTGGCAAAAAGGATATTTTAAGTCGAACTGGAAGGGGGGCCGGGCAGcttct > 2:961227/1‑90 (MQ=255) cAGCCATTGCGGCGGGGCTGTCGATGGGGGCCTCGTTTCTTGCAAAAAGGGAATTTTAAATCGGACTGGGAGGGGGGCCGGGCAGcttct > 1:694763/1‑90 (MQ=255) gCCATTGCGGCGGGGCTGTCGATGGGGGCTTCGTTACTGGCAAAAAGGGTATTTTAAGTCGAACTGGAAGGGGGGCCGGGGAGcttcttt > 2:877351/1‑89 (MQ=255) gggTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAAtct < 1:314019/90‑1 (MQ=255) tCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTtat < 2:142427/90‑1 (MQ=255) cgcTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTgg < 2:1050860/90‑1 (MQ=255) | GAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGG > NZ_CP009273/2458903‑2459081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |